조재열 (성균관대학교)

한빛사논문

조회200

성균관대학교

CV File 584 kb

Adv. Healthc. Mater., Aug 01 2024, | https://doi.org/10.1002/adhm.202401466 Oral Piwi-Interacting RNA Delivery Mediated by Green Tea-Derived Exosome-Like Nanovesicles for the Treatment of Aortic Dissection

Yan Liu ^1,2, Hongzhao Qi ¹, Jinbao Zong ³, Min Li ³, Yanyan Yang⁴, Xiaolu Li ⁵, Tianxiang Li ¹, Jae Youl Cho ², Tao Yu ^1,5

¹Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, No. 38 Dengzhou Road, Qingdao, 266021, P. R. China.

²Department of Integrative Biotechnology, Sungkyunkwan University, 300 Chuncheon-Dong, Suwon, 16419, Republic of Korea.

³Clinical Laboratory, Central Laboratory, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital), Qingdao, 266000, P. R. China.

⁴Department of Immunology, School of Basic Medicine, Qingdao University, Qingdao, 266021, P. R. China.

⁵Department of Cardiac Ultrasound, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, P. R. China.

Y.L. and H.Q. contributed equally to this work.

Corresponding Authors: Jae Youl Cho, Tao Yu

Abstract

Aortic dissection (AD) is a severe cardiovascular disease necessitating active therapeutic strategies for early intervention and prevention. Nucleic acid drugs, known for their potent molecule-targeting therapeutic properties, offer potential for genetic suppression of AD. Piwi-interacting RNAs, a class of small RNAs, hold promise for managing cardiovascular diseases. Limited research on these RNAs and AD exists. This study demonstrates that an antagomir targeting heart-apoptosis-associated piRNA (HAAPIR) effectively regulates vascular remodeling, mitigating AD occurrence and progression through the myocyte enhancer factor 2D (Mef2D) and matrix metallopeptidase 9 (MMP9) pathways. Green tea-derived plant exosome-like nanovesicles (PELNs) are used for oral administration of antagomir. The antagomir-HAAPIR-nanovesicle complex, after purification and optimization, exhibits a high packing rate, while the antagomir is resistant to enzyme digestion. Administered to mice, the complex targets the aortic lesion, reducing AD incidence and improving survival. Moreover, MMP9 and Mef2D expression decrease significantly, inhibiting the phenotypic conversion of human aortic smooth muscle cells. PELNs encapsulate the antagomir-HAAPIR complex, maintaining stability, mediating transport into the bloodstream, and delivering Piwi-interacting RNAs to AD sites. Thus, HAAPIR is a potential target for persistent clinical AD prevention and treatment, and nanovesicle-encapsulated nucleic acids offer a promising cardiovascular disease treatment, providing insights for other therapeutic targets.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-05)
연구진국내(교신)+국외 연구진
분야 생명과학 > 면역학

댓글 작성 로그인

CV 열람하기

연락처 보기