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Adv. Mater., Jul 31 2024, | https://doi.org/10.1002/adma.202311931 Ultrafast Metaphotonic PCR Chip with Near-Perfect Absorber

Inki Kim ^1,2, Hongyoon Kim ³, Myeongcheol Go ⁴, Seho Lee ^1,2, Dang Du Nguyen ^1,2, Seongryeong Kim ^1,2, Kiran Shrestha ^1,2, Abdulrahman Alsaadi ^1,2, Youngsun Jeon ³, Sebin Jeong⁴, Gyoujin Cho ^1,2, Jin Kon Kim⁴, Junsuk Rho^3,4,5,6,7, Luke P. Lee^1,8,9

¹Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

²Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

³Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

⁴Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

⁵Department of Electrical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

⁶POSCO-POSTECH-RIST Convergence Research Center for Flat Optics and Metaphotonics, Pohang, 37673, Republic of Korea.

⁷National Institute of Nanomaterials Technology (NINT), Pohang, 37673, Republic of Korea.

⁸Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.

⁹Department of Bioengineering, Department of Electrical Engineering and Computer Science, University of California, Berkeley, CA, 94720, USA.

I.K., H.K., and M.G. contributed equally to this work.

Corresponding Authors: Inki Kim, Jin Kon Kim, Junsuk Rho, Luke P. Lee

Abstract

Polymerase chain reaction (PCR) is the gold standard for nucleic acid amplification and quantification in diverse fields such as life sciences, global health, medicine, agricultural science, forensic science, and environmental science for global sustainability. However, implementing a cost-effective PCR remains challenging for rapid preventive medical action to the widespread pandemic diseases due to the absence of highly efficient and low-cost PCR chip-based POC molecular diagnostics. Here, this work reports an ultrafast metaphotonic PCR chip as a solution of a cost-effective and low-power-consumption POC device for the emerging global challenge of sustainable healthcare. This work designs a near-perfect photonic meta-absorber using ring-shaped titanium nitride to maximize the photothermal effect and realize rapid heating and cooling cycles during the PCR process. This work fabricates a large-area photonic meta-absorber on a 6-inch wafer cost-effectively using simple colloidal lithography. In addition, this work demonstrates 30 thermocycles from 65 (annealing temperature) to 95 °C (denaturation temperature) within 3 min 15 s, achieving an average 16.66 °C s^-1 heating rate and 7.77 °C s^-1 cooling rate during thermocycling, succeeding rapid metaphotonic PCR. This work believes a metaphotonic PCR chip can be used to create a low-cost, ultrafast molecular diagnostic chip with a meta-absorber.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-08-05)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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