김경호 (성균관대학교)

한빛사논문

조회115

김경호Kyung Ho Kim

성균관대학교

J. Hazard. Mater., Jul 21 2024, 477 S0304-3894(24)01861-2 | https://doi.org/10.1016/j.jhazmat.2024.135282 TRPA1 nanovesicle-conjugated receptonics for rapid biocide screening

Kyung Ho Kim ^a,1, Jisung Kwak ^b,c,1, Sung Eun Seo ^a, Siyoung Ha ^d, Gyeong-Ji Kim ^a, Sangwoo Lee^e,g, Sang Jun Sim ^c, Yeon Kyung Lee ^b,f, Na Ly Tran^h, Seung Ja Oh ^h, Woo-Keun Kim ^e,g, Hyun Seok Song ^b, Oh Seok Kwon^a,i,j

^aSKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Republic of Korea

^bSensor System Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea

^cDepartment of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea

^dInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea

^eDepartment of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea

^fDepartment of Biomicrosystem Technology, Korea University, Seoul, 02841, Republic of Korea

^gHuman and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea

^hDepartment of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Gyeonggi-do, Republic of Korea

ⁱDepartment of Nano Science and Technology, Sungkyunkwan University, Suwon 16419, Republic of Korea

^jDepartment of Nano Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea

¹These authors contributed equally to this work.

Corresponding authors: Woo-Keun Kim, Hyun Seok Song, Oh Seok Kwon

Abstract

Although biocides are important materials in modern society and help protect human health and the environment, increasing exposure to combined biocides can cause severe side effects in the human body, such as lung fibrosis. In this study, we developed a receptonics system to screen for biocides in combined household chemical products based on biocides. The system contains transient receptor potential ankyrin 1 (TRPA1) nanovesicles (NVs) to sense biocides based on pain receptors and a side-gated field-effect transistor (SGFET) using a single-layer graphene (SLG) micropattern channel. The binding affinities between the TRPA1 receptor and the various biocides were estimated by performing biosimulation and using a calcium ion (Ca²⁺) assay, and the sensitivity of the system was compared with that of TRPA1 NV receptonics systems. Based on the results of the TRPA1 NV receptonics system, the antagonistic and potentiation effects of combined biocides and household chemical products depended on the concentration. Finally, the TRPA1 NV receptonics system was applied to screen for biocides in real products, and its performance was successful. Based on these results, the TRPA1 NV receptonics system can be utilized to perform risk evaluations and identify biocides in a simple and rapid manner.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-08-05)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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