임종원 (University of Illinois at Urbana-Champaign)

한빛사논문

조회96

University of Illinois at Urbana-Champaign

CV File 78 kb

Adv. Healthc. Mater., Jul 29 2024, | https://doi.org/10.1002/adhm.202402506 Dried Blood Matrix as a New Material for the Detection of DNA Viruses

Jongwon Lim ^1,2,3, Joanne Hwang ^3,4, Hyegi Min ¹, Matthew Wester ^1,2, Chansong Kim ⁵, Enrique Valera ^1,2, Hyun Joon Kong^3,4,6, Rashid Bashir ^{1,2,3,5,7,8,9}

¹Nick Holonyak Jr. Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

²Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

³Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁴Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁵Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁶Departments of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁷Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁸Department of Biomedical and Translational Science, Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

⁹Chan Zuckerberg Biohub Chicago, Chicago, IL, 60642, USA.

Corresponding Author : Rashid Bashir

Abstract

The gold standard for diagnosing viruses such as the Hepatitis B Virus has remained largely unchanged, relying on conventional methods involving extraction, purification, and polymerase chain reaction (PCR). This approach is hindered by limited availability, as it is time-consuming and requires highly trained personnel. Moreover, it suffers from low recovery rates of the nucleic acid molecules for samples with low copy numbers. To address the challenges of complex instrumentation and low recovery rate of DNA, a drying process coupled with thermal treatment of whole blood is employed, resulting in the creation of a dried blood matrix characterized by a porous structure with a high surface-to-volume ratio where it also inactivates the amplification inhibitors present in whole blood. Drawing on insights from Brunauer-Emmett-Teller (BET)- Barrett-Joyner-Halenda (BJH) analysis, scanning electron microscopy (SEM), and fluorescence recovery after photobleaching (FRAP), detection assay is devised for HBV, as a demonstration, from whole blood with high recovery of DNA and simplified instrumentation achieving a limit of detection (LOD) of 10 IU mL^-1. This assay can be completed in <1.5 h using a simple heater, can be applied to other DNA viruses, and is expected to be suitable for point-of-care, especially in low-resource settings.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-08-03)
연구진국외 연구진
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