김정은 (University of Hong Kong (현 Weill Cornell Graduate School, Cornell University))

한빛사논문

조회244

University of Hong Kong (현 Weill Cornell Graduate School, Cornell University)

CV File 277 kb

Nucleic Acids Res., 01 August 2024 | https://doi.org/10.1093/nar/gkae661 PHF8 facilitates transcription recovery following DNA double-strand break repair

Jung Eun Kim ¹, Xiyue Pan ¹, Kwan Yiu Tse ¹, Henry Hei Chan ¹, Chao Dong ^1,2 and Michael Shing Yan Huen^1,*

¹School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong S.A.R.
²Department of Occupational and Environmental Health, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China

^*Corresponding author: correspondence to Michael Shing Yan Huen

Abstract

Transient halting of transcription activity on the damaged chromatin facilitates DNA double-strand break (DSB) repair. However, the molecular mechanisms that facilitate transcription recovery following DSB repair remain largely undefined. Notably, failure to restore gene expression in a timely manner can compromise transcriptome signatures and may impose deleterious impacts on cell identity and cell fate. Here, we report PHF8 as the major demethylase that reverses transcriptionally repressive epigenetic modification laid down by the DYRK1B–EHMT2 pathway. We found that PHF8 concentrates at laser-induced DNA damage tracks in a DYRK1B-dependent manner and promotes timely resolution of local H3K9me2 to facilitate the resumption of transcription. Moreover, PHF8 also assists in the recovery of ribosomal DNA (rDNA) transcription following the repair of nucleolar DSBs. Taken together, our findings uncover PHF8 as a key mediator that coordinates transcription activities during the recovery phase of DSB responses.

논문정보

형식Research article
게재일2024년 08월 (BRIC 등록일 2024-08-06)
연구진국외 연구진
분야 생명과학 > 세포생물학

댓글 작성 로그인

CV 열람하기

연락처 보기