임기택 (강원대학교)

한빛사논문

조회112

강원대학교

Biomaterials, Jul 26 2024, 312 S0142-9612(24)00247-3 | https://doi.org/10.1016/j.biomaterials.2024.122713 Transcriptomic profiling of human mesenchymal stem cells using a pulsed electromagnetic-wave motion bioreactor system for enhanced osteogenic commitment and therapeutic potentials

Aayushi Randhawa^a,b,1, Keya Ganguly ^a,c,1, Sayan Deb Dutta^a, Tejal V. Patil ^a,b, Ki-Taek Lim^a,b,c

^aDepartment of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea

^bInterdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, 24341, Republic of Korea

^cInstitute of Forest Science, Kangwon National University, Chuncheon, 24341, Republic of Korea

¹These authors have contributed equally to the manuscript.

Corresponding author : Ki-Taek Lim

Abstract

Traditional bioreactor systems involve the use of three-dimensional (3D) scaffolds or stem cell aggregates, limiting the accessibility to the production of cell-secreted biomolecules. Herein, we present the use a pulse electromagnetic fields (pEMFs)-assisted wave-motion bioreactor system for the dynamic and scalable culture of human bone marrow-derived mesenchymal stem cells (hBMSCs) with enhanced the secretion of various soluble factors with massive therapeutic potential. The present study investigated the influence of dynamic pEMF (D-pEMF) on the kinetic of hBMSCs. A 30-min exposure of pEMF (10V-1Hz, 5.82 G) with 35 oscillations per minute (OPM) rocking speed can induce the proliferation (1 × 10⁵ → 4.5 × 10⁵) of hBMSCs than static culture. Furthermore, the culture of hBMSCs in osteo-induction media revealed a greater enhancement of osteogenic transcription factors under the D-pEMF condition, suggesting that D-pEMF addition significantly boosted hBMSCs osteogenesis. Additionally, the RNA sequencing data revealed a significant shift in various osteogenic and signaling genes in the D-pEMF group, further suggesting their osteogenic capabilities. In this research, we demonstrated that the combined effect of wave and pEMF stimulation on hBMSCs allows rapid proliferation and induces osteogenic properties in the cells. Moreover, our study revealed that D-pEMF stimuli also induce ROS-scavenging properties in the cultured cells. This study also revealed a bioactive and cost-effective approach that enables the use of cells without using any expensive materials and avoids the possible risks associated with them post-implantation.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-08-05)
연구진국내 연구진
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