김정원 (POSTECH)

한빛사논문

조회155

POSTECH

CV File 196 kb

Adv. Sci., Jul 29 2024, | https://doi.org/10.1002/advs.202402029 START: A Versatile Platform for Bacterial Ligand Sensing with Programmable Performances

Jeongwon Kim¹, Minchae Seo¹, Yelin Lim¹, Jongmin Kim ¹

¹Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea.

J.K. and M.S. contributed equally to this work.

Corresponding Author : Jongmin Kim

Abstract

Recognition of signaling molecules for coordinated regulation of target genes is a fundamental process for biological systems. Cells often rely on transcription factors to accomplish these intricate tasks, yet the subtle conformational changes of protein structures, coupled with the complexity of intertwined protein interaction networks, pose challenges for repurposing these for bioengineering applications. This study introduces a novel platform for ligand-responsive gene regulation, termed START (Synthetic Trans-Acting Riboswitch with Triggering RNA). Inspired by the bacterial ligand sensing system, riboswitch, and the synthetic gene regulator, toehold switch, the START platform enables the implementation of synthetic biosensors for various ligands. Rational sequence design with targeted domain optimization yields high-performance STARTs with a dynamic range up to 67.29-fold and a tunable ligand sensitivity, providing a simple and intuitive strategy for sensor engineering. The START platform also exhibits modularity and composability to allow flexible genetic circuit construction, enabling seamless implementation of OR, AND, and NOT Boolean logic gates for multiple ligand inputs. The START design principle is capable of broadening the suite of synthetic biosensors for diverse chemical and protein ligands, providing a novel riboregulator chassis for synthetic biology and bioengineering applications.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-31)
연구진국내 연구진
분야 생명과학 > 생물공학

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