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Max-Planck Institute for Plant Breeding Research

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New Phytol., Jul 26 2024, | https://doi.org/10.1111/nph.19981 Contrasting and conserved roles of NPR pathways in diverged land plant lineages

Hyung-Woo Jeon^1*, Hidekazu Iwakawa^1,2*, Satoshi Naramoto^3,4, Cornelia Herrfurth^5,6, Nora Gutsche⁷, Titus Schlüter¹, Junko Kyozuka³, Shingo Miyauchi¹, Ivo Feussner^5,6, Sabine Zachgo⁷ and Hirofumi Nakagami¹

¹Max- Planck Institute for Plant Breeding Research, 50829, Cologne, Germany;

²School of Biological Science and Technology, College of Science and Engineering, Kanazawa University, Kakuma- machi, Kanazawa, 920-1192, Japan;

³Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan;

⁴Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan;

⁵Service Unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, 37077, Göttingen, Germany;

⁶Department for Plant Biochemistry, Albrecht von Haller Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, 37077, Göttingen, Germany;

⁷Division of Botany, Osnabrück University, 49076, Osnabrück, Germany

^*These authors contributed equally to this work.

Author for correspondence: Hirofumi Nakagami

Abstract

The NPR proteins function as salicylic acid (SA) receptors in Arabidopsis thaliana. AtNPR1 plays a central role in SA-induced transcriptional reprogramming whereby positively regulates SA-mediated defense. NPRs are found in the genomes of nearly all land plants. However, we know little about the molecular functions and physiological roles of NPRs in most plant species. We conducted phylogenetic and alignment analyses of NPRs from 68 species covering the significant lineages of land plants. To investigate NPR functions in bryophyte lineages, we generated and characterized NPR loss-of-function mutants in the liverwort Marchantia polymorpha. Brassicaceae NPR1-like proteins have characteristically gained or lost functional residues identified in AtNPRs, pointing to the possibility of a unique evolutionary trajectory for the Brassicaceae NPR1-like proteins. We find that the only NPR in M. polymorpha, MpNPR, is not the master regulator of SA-induced transcriptional reprogramming and negatively regulates bacterial resistance in this species. The Mpnpr transcriptome suggested roles of MpNPR in heat and far-red light responses. We identify both Mpnpr and Atnpr1-1 display enhanced thermomorphogenesis. Interspecies complementation analysis indicated that the molecular properties of AtNPR1 and MpNPR are partially conserved. We further show that MpNPR has SA-binding activity. NPRs and NPR-associated pathways have evolved distinctively in diverged land plant lineages to cope with different terrestrial environments.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-31)
연구진국외 연구진
분야 생명과학 > 식물학

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