한빛사논문
- 조회125
한림대학교
Satya Priya Sharma1, Haripriya Gupta1, Goo-Hyun Kwon1, Sang Yoon Lee1, Seol Hee Song1, Jeoung Su Kim1, Jeong Ha Park1, Min Ju Kim1, Dong-Hoon Yang1, Hyunjoon Park1, Sung-Min Won1, Jin-Ju Jeong1, Ki-Kwang Oh1, Jung A Eom1, Kyeong Jin Lee1, Sang Jun Yoon1, Young Lim Ham2, Gwang Ho Baik1,3, Dong Joon Kim1,3, Ki Tae Suk1,3
1Institute for Liver and Digestive Diseases, Hallym University, Chuncheon, Republic of Korea 24253
2Department of Nursing Daewon University College Jecheon, Republic of Korea 27135
3Department of Internal Medicine, Hallym University College of Medicine, Chuncheon, Republic of Korea 24253
Correspondence : Ki Tae Suk
*Satya Priya Sharma and Jeoung Su Kim contributed equally to this work.
Abstract
Background/Aims
Shifts in the gut microbiota and metabolites are interrelated with liver cirrhosis progression and complications. However, causal relationships have not been evaluated comprehensively. Here, we identified complication-dependent gut microbiota and metabolic signatures in patients with liver cirrhosis.
Methods
Microbiome taxonomic profiling was performed on 194 stool samples (52 controls and 142 cirrhosis patients) via V3-V4 16S rRNA sequencing. Next, 51 samples (17 controls and 34 cirrhosis patients) were selected for fecal metabolite profiling via gas chromatography mass spectrometry and liquid chromatography coupled to time-of-flight–mass spectrometry. Correlation analyses were performed targeting the gut- microbiota, metabolites, clinical parameters, and presence of complications (varices, ascites, peritonitis, encephalopathy, hepatorenal syndrome, hepatocellular carcinoma, and deceased).
Results
Veillonella bacteria, Ruminococcus gnavus, and Streptococcus pneumoniae are cirrhosis-related microbiotas compared with control group. Bacteroides ovatus, Clostridium symbiosum, Emergencia timonensis, Fusobacterium varium, and Hungatella_uc were associated with complications in the cirrhosis group. The areas under the receiver operating characteristic curve (AUROCs) for the diagnosis of cirrhosis, encephalopathy, hepatorenal syndrome, and deceased were 0.863, 0.733, 0.71, and 0.69, respectively. The AUROCs of mixed microbial species for the diagnosis of cirrhosis and complication were 0.808 and 0.847, respectively. According to the metabolic profile, 5 increased fecal metabolites in patients with cirrhosis were biomarkers (AUROC > 0.880) for the diagnosis of cirrhosis and complications. Clinical markers were significantly correlated with the gut microbiota and metabolites.
Conclusion
Cirrhosis-dependent gut microbiota and metabolites present unique signatures that can be used as noninvasive biomarkers for the diagnosis of cirrhosis and its complications.
논문정보
- Research article
- 2024년 07월 (BRIC 등록일 2024-07-29)
- 국내 연구진
- 의약학 > 응용의학
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