조서현 (Norwich Research Park)

한빛사논문

조회77

Norwich Research Park

Nat. Chem. Biol., Jul 23 2024, | https://doi.org/10.1038/s41589-024-01681-7 Unlocking saponin biosynthesis in soapwort

Seohyun Jo¹, Amr El-Demerdash^1,2, Charlotte Owen¹, Vikas Srivastava^1,3, Dewei Wu¹, Shingo Kikuchi¹, James Reed¹, Hannah Hodgson¹, Alex Harkess⁴, Shengqiang Shu⁵, Chris Plott⁴, Jerry Jenkins⁴, Melissa Williams⁴, Lori-Beth Boston⁴, Elia Lacchini^6,7, Tongtong Qu^6,7, Alain Goossens^6,7, Jane Grimwood⁴, Jeremy Schmutz^4,5, Jim Leebens-Mack⁸ & Anne Osbourn¹

¹Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich, UK.

²Department of Chemistry, Faculty of Sciences, Mansoura University, Mansoura, Egypt.

³Department of Botany, School of Life Sciences, Central University of Jammu, Jammu, India.

⁴HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA.

⁵US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

⁶Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.

⁷VIB Centre for Plant Systems Biology, Ghent, Belgium.

⁸Department of Plant Biology, Miller Plant Sciences, University of Georgia, Athens, GA, USA.

Corresponding author

Correspondence to Anne Osbourn.

Abstract

Soapwort (Saponaria officinalis) is a flowering plant from the Caryophyllaceae family with a long history of human use as a traditional source of soap. Its detergent properties are because of the production of polar compounds (saponins), of which the oleanane-based triterpenoid saponins, saponariosides A and B, are the major components. Soapwort saponins have anticancer properties and are also of interest as endosomal escape enhancers for targeted tumor therapies. Intriguingly, these saponins share common structural features with the vaccine adjuvant QS-21 and, thus, represent a potential alternative supply of saponin adjuvant precursors. Here, we sequence the S. officinalis genome and, through genome mining and combinatorial expression, identify 14 enzymes that complete the biosynthetic pathway to saponarioside B. These enzymes include a noncanonical cytosolic GH1 (glycoside hydrolase family 1) transglycosidase required for the addition of D-quinovose. Our results open avenues for accessing and engineering natural and new-to-nature pharmaceuticals, drug delivery agents and potential immunostimulants.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-26)
연구진국외 연구진
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