조혜원 (고려대학교)

한빛사논문

조회312

고려대학교

J. Integr. Plant Biol., Jul 23 2024, | https://doi.org/10.1111/jipb.13747 SERKs serve as co-receptors for SYR1 to trigger systemin-mediated defense responses in tomato

Hyewon Cho^1†, Dain Seo^1†, Minsoo Kim¹, Bo Eun Nam^2,3, Soyoun Ahn¹, Minju Kang¹, Geul Bang⁴, Choon‐Tak Kwon⁵, Youngsung Joo³ and Eunkyoo Oh^1*

¹Department of Life Sciences, Korea University, Seoul 02841, Korea

²Research, Institute of Basic Sciences, Seoul National University, Seoul 08826, Korea

³School of Biological Sciences, Seoul National University, Seoul 08826, Korea

⁴Digital Omics Research Center, Ochang Institute of Biological and Environmental Science, Korea Basic Science Institute, Cheongju28119, Korea

⁵Department of Smart Farm Science, Kyung Hee University, Yongin 17104, Korea

^†These authors contributed equally to this work.

^*Correspondence: Eunkyoo Oh

Abstract

Systemin, the first peptide hormone identified in plants, was initially isolated from tomato (Solanum lycopersicum) leaves. Systemin mediates local and systemic wound-induced defense responses in plants, conferring resistance to necrotrophic fungi and herbivorous insects. Systemin is recognized by the leucine-rich-repeat receptor-like kinase (LRR-RLK) receptor SYSTEMIN RECEPTOR1 (SYR1), but how the systemin recognition signal is transduced to intracellular signaling pathways to trigger defense responses is poorly understood. Here, we demonstrate that SERK family LRR-RLKs function as co-receptors for SYR1 to mediate systemin signal transduction in tomato. By using chemical genetic approaches coupled with engineered receptors, we revealed that the association of the cytoplasmic kinase domains of SYR1 with SERKs leads to their mutual trans-phosphorylation and the activation of SYR1, which in turn induces a wide range of defense responses. Systemin stimulates the association between SYR1 and all tomato SERKs (SlSERK1, SlSERK3A, and SlSERK3B). The resulting SYR1-SlSERK heteromeric complexes trigger the phosphorylation of TOMATO PROTEIN KINASE 1B (TPK1b), a receptor-like cytoplasmic kinase that positively regulates systemin responses. Additionally, upon association with SYR1, SlSERKs are cleaved by the Pseudomonas syringae effector HopB1, further supporting the finding that SlSERKs are activated by systemin-bound SYR1. Finally, genetic analysis using Slserk mutants showed that SlSERKs are essential for systemin-mediated defense responses. Collectively, these findings demonstrate that the systemin-mediated association of SYR1 and SlSERKs activates defense responses against herbivorous insects.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-24)
연구진국내 연구진
분야 생명과학 > 분자생물학

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