박지원 (Weill Cornell Medicine (Cornell University) and Harvard Medical School)

한빛사논문

조회269

Weill Cornell Medicine (Cornell University) and Harvard Medical School

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Nat. Commun., Jun 11 2024, 15 (1) 4773 | https://doi.org/10.1038/s41467-024-48625-2 Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight

Jiwoon Park ^1,2, Eliah G. Overbey ^1,2, S. Anand Narayanan ₃, JangKeun Kim ^1,2, Braden T. Tierney ^1,2, Namita Damle ¹, Deena Najjar ¹, Krista A. Ryon ¹, Jacqueline Proszynski ¹, Ashley Kleinman ¹, Jeremy Wain Hirschberg ¹, Matthew MacKay ¹, Evan E. Afshin ¹, Richard Granstein ⁴, Justin Gurvitch ¹, Briana M. Hudson ⁵, Aric Rininger ⁵, Sean Mullane ⁶, Sarah E. Church ⁵, Cem Meydan^1,2, George Church ^7,8, Afshin Beheshti ^9,10, Jaime Mateus ⁶ & Christopher E. Mason^1,2,11,*

¹Department of Physiology, Biophysics and Systems Biology, Weill Cornell Medicine, New York, NY, USA.
²The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.
³Department of Nutrition & Integrative Physiology, Florida State University, Tallahassee, FL, USA.
⁴Department of Dermatology, Weill Cornell Medicine, New York, NY, USA.
⁵NanoString Technologies, Inc., Seattle, WA, USA.
⁶SpaceX, Hawthorne, CA, USA.
⁷Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
⁸Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
⁹Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
¹⁰Blue Marble Space Institute of Science, Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.
¹¹The Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.

^*Corresponding author: correspondence to Christopher E. Mason

Abstract

Spaceflight can change metabolic, immunological, and biological homeostasis and cause skin rashes and irritation, yet the molecular basis remains unclear. To investigate the impact of short-duration spaceflight on the skin, we conducted skin biopsies on the Inspiration4 crew members before (L-44) and after (R + 1) flight. Leveraging multi-omics assays including GeoMx™ Digital Spatial Profiler, single-cell RNA/ATAC-seq, and metagenomics/metatranscriptomics, we assessed spatial gene expressions and associated microbial and immune changes across 95 skin regions in four compartments: outer epidermis, inner epidermis, outer dermis, and vasculature. Post-flight samples showed significant up-regulation of genes related to inflammation and KRAS signaling across all skin regions. These spaceflight-associated changes mapped to specific cellular responses, including altered interferon responses, DNA damage, epithelial barrier disruptions, T-cell migration, and hindered regeneration were located primarily in outer tissue compartments. We also linked epithelial disruption to microbial shifts in skin swab and immune cell activity to PBMC single-cell data from the same crew and timepoints. Our findings present the inaugural collection and examination of astronaut skin, offering insights for future space missions and response countermeasures.

논문정보

형식Research article
게재일2024년 06월 (BRIC 등록일 2024-07-30)
연구진국외 연구진
분야 의약학 > 인체시스템의학

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