이지영 (The Ohio State University) | 한빛사논문 > 한빛사

한빛사논문

조회354

이지영Jiyoung Lee

The Ohio State University

CV File 318 kb

Environ. Sci. Technol. , Jul 16 2024, | https://doi.org/10.1021/acs.est.3c09070 One Health Threat of Treated Wastewater Discharge in Urban Ohio Rivers: Implications for Surface Water and Fish Gut Microbiome and Resistome

Molly Mills¹, Dixie Mollenkopf ², Thomas Wittum ^2,3, Mažeika Patricio Sullivan ⁴, Jiyoung Lee^1,3,5

¹Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, Ohio 43210, United States.

²Department of Veterinary Preventive Medicine, The Ohio State University, Columbus, Ohio 43210, United States.

³Infectious Diseases Institute, The Ohio State University, Columbus, Ohio 43210, United States.

⁴Baruch Institute of Coastal Ecology & Forest Science, Clemson University, Georgetown, South Carolina 29442, United States.

⁵Department of Food Science & Technology, The Ohio State University, Columbus, Ohio 43210, United States.

Corresponding Author : Jiyoung Lee

Abstract

Wastewater treatment plants (WWTPs) are thought to be a major disseminating source of antibiotic resistance (AR) to the environment, establishing a crucial connection between human and environmental resistome. The objectives of this study were to determine how wastewater effluents impact microbiome and resistome of freshwater and fish, and identify potential AR-carrying clinically relevant pathogens in these matrices. We analyzed wastewater influent and effluent from four WWTPs in three metropolitan areas of Ohio, USA via shotgun metagenomic sequencing. We also sequenced river water and fish guts from three reaches (upstream, at the WWTP outfall, and downstream). Notably, we observed a decline in microbiome diversity and AR gene abundance from wastewater to the receiving river. We also found significant differences by reach and trophic level (diet) in beta-diversity of the fish gut microbiomes. SourceTracker revealed that 0.443 and 0.248 more of the of the fish gut microbiome was sourced from wastewater effluent in fish from the outfall and downstream locations, respectively, compared to upstream fish. Additionally, AR bacteria of public health concern were annotated in effluent and river water samples, indicating potential concern for human exposure. In summary, our findings show the continued role of wastewater as a significant AR reservoir and underscores the considerable impact of wastewater discharge on aquatic wildlife, which highlights the One Health nature of this issue.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-21)
연구진국외 연구진
분야 생명과학 > 미생물학

댓글 작성 로그인

CV 열람하기

연락처 보기