조영학 (KIST, KAIST)

한빛사논문

조회199

KIST, KAIST

Adv. Mater., Jul 17 2024, | https://doi.org/10.1002/adma.202403952 Long-Term Culture of Human Pluripotent Stem Cells in Xeno-Free Condition Using Functional Polymer Films

Younghak Cho ^1,2, Hana Lee ³, Wonji Jeong², Kwang Bo Jung ³, Sun Young Lee⁴, Seonghyeon Park², Jemin Yeun², Ohman Kwon ³, Jin Gyeong Son ⁴, Tae Geol Lee ⁴, Mi-Young Son ^3,5,6, Sung Gap Im²

¹Brain Science Institute, Korea Institute of Science and Technology (KIST), 5, Hwarang-ro 14-gil, Seongbuk-gu, Seoul, 02792, Republic of Korea.

²Functional Thin Film Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

³Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.

⁴Korea Research Institute of Standards and Science (KRISS), Daejeon, 34141, Republic of Korea.

⁵KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.

⁶Department of Biological Science, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

Y.C., H.L., and W.J. contributed equally to this work

CORRESPONDING AUTHORS: Mi-Young Son, Sung Gap Im

Abstract

Human pluripotent stem cells (hPSCs), encompassing human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), hold immense potential in regenerative medicine, offering new opportunities for personalized cell therapies. However, their clinical translation is hindered by the inevitable reliance on xenogeneic components in culture environments. This study addresses this challenge by engineering a fully synthetic, xeno-free culture substrate, whose surface composition is tailored systematically for xeno-free culture of hPSCs. A functional polymer surface, pGC2 (poly(glycidyl methacrylate-grafting-guanidine-co-carboxylic acrylate)), offers excellent cell-adhesive properties as well as non-cytotoxicity, enabling robust hESCs and hiPSCs growth while presenting cost-competitiveness and scalability over Matrigel. This investigation includes comprehensive evaluations of pGC2 across diverse experimental conditions, demonstrating its wide adaptability with various pluripotent stem cell lines, culture media, and substrates. Crucially, pGC2 supports long-term hESCs and hiPSCs expansion, up to ten passages without compromising their stemness and pluripotency. Notably, this study is the first to confirm an identical proteomic profile after ten passages of xeno-free cultivation of hiPSCs on a polymeric substrate compared to Matrigel. The innovative substrate bridges the gap between laboratory research and clinical translation, offering a new promising avenue for advancing stem cell-based therapies.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-18)
연구진국내 연구진
분야 바이오·의료융합 > 바이오소재

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