김정우 (University of California, Berkeley) | 한빛사논문 > 한빛사

한빛사논문

조회463

University of California, Berkeley

CV File 115 kb

Nat. Chem. Biol., Jul 2024, 20 (7) | https://doi.org/10.1038/s41589-024-01603-7 Molecular recording of calcium signals via calcium-dependent proximity labeling

J. Wren Kim¹, Adeline J. H. Yong^2,3,8, Erin E. Aisenberg^4,8, Joseph H. Lobel¹, Wei Wang⁵, Ted M. Dawson⁶, Valina L. Dawson⁶, Ruixuan Gao⁵, Yuh Nung Jan^2,3, Helen S. Bateup^1,4,7& Nicholas T. Ingolia¹

¹Department of Molecular and Cell Biology at the University of California, Berkeley, Berkeley, CA, USA.

²Department of Physiology at the University of California, San Francisco, San Francisco, CA, USA.

³Howard Hughes Medical Institute at the University of California, San Francisco, San Francisco, CA, USA.

⁴Helen Wills Neuroscience Institute at the University of California, Berkeley, Berkeley, CA, USA.

⁵Department of Chemistry at the University of Illinois, Chicago, Chicago, IL, USA. \

⁶Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

⁷Chan Zuckerberg Biohub, San Francisco, CA, USA.

⁸These authors contributed equally: Adeline J. H. Yong, Erin E. Aisenberg.

Corresponding author

Correspondence to Nicholas T. Ingolia.

Abstract

Calcium ions serve as key intracellular signals. Local, transient increases in calcium concentrations can activate calcium sensor proteins that in turn trigger downstream effectors. In neurons, calcium transients play a central role in regulating neurotransmitter release and synaptic plasticity. However, it is challenging to capture the molecular events associated with these localized and ephemeral calcium signals. Here we present an engineered biotin ligase that generates permanent molecular traces in a calcium-dependent manner. The enzyme, calcium-dependent BioID (Cal-ID), biotinylates nearby proteins within minutes in response to elevated local calcium levels. The biotinylated proteins can be identified via mass spectrometry and visualized using microscopy. In neurons, Cal-ID labeling is triggered by neuronal activity, leading to prominent protein biotinylation that enables transcription-independent activity labeling in the brain. In summary, Cal-ID produces a biochemical record of calcium signals and neuronal activity with high spatial resolution and molecular specificity.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-18)
연구진국외 연구진
분야 생명과학 > 생화학

댓글 작성 로그인

CV 열람하기

연락처 보기