송은호 (서울대학교, 현 NIH) | 한빛사논문 > 한빛사

한빛사논문

조회680

서울대학교, 현 NIH

CV File 291 kb

Nucleic Acids Res., Jul 16 2024, gkae620 | https://doi.org/10.1093/nar/gkae620 Single-mode termination of phage transcriptions, disclosing bacterial adaptation for facilitated reinitiations

Eunho Song^1,†, Sun Han ^1,†, Heesoo Uhm ¹, Changwon Kang^2,* and Sungchul Hohng ^1,*

¹Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 08826, Republic of Korea

²Department of Biological Sciences, and KAIST Stem Cell Center, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea

^*To whom correspondence should be addressed. : Sungchul Hohng

Correspondence may also be addressed to Changwon Kang.

^†The first two authors should be regarded as Joint First Authors.

Present addresses:

Eunho Song, Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Heesoo Uhm, Department of Biochemistry and Molecular Biology, and Medical Research Center of Genomic Medicine Institute, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.

Abstract

Bacterial and bacteriophage RNA polymerases (RNAPs) have divergently evolved and share the RNA hairpin-dependent intrinsic termination of transcription. Here, we examined phage T7, T3 and SP6 RNAP terminations utilizing the single-molecule fluorescence assays we had developed for bacterial terminations. We discovered the phage termination mode or outcome is virtually single with decomposing termination. Therein, RNAP is displaced forward along DNA and departs both RNA and DNA for one-step decomposition, three-dimensional diffusion and reinitiation at any promoter. This phage displacement-mediated decomposing termination is much slower than readthrough and appears homologous with the bacterial one. However, the phage sole mode of termination contrasts with the bacterial dual mode, where both decomposing and recycling terminations occur compatibly at any single hairpin- or Rho-dependent terminator. In the bacterial recycling termination, RNA is sheared from RNA·DNA hybrid, and RNAP remains bound to DNA for one-dimensional diffusion, which enables facilitated recycling for reinitiation at the nearest promoter located downstream or upstream in the sense or antisense orientation. Aligning with proximity of most terminators to adjacent promoters in bacterial genomes, the shearing-mediated recycling termination could be bacterial adaptation for the facilitated reinitiations repeated at a promoter for accelerated expression and coupled at adjoining promoters for coordinated regulation.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-17)
연구진국내 연구진
분야 생명과학 > 구조생물 및 생물물리학

댓글 작성 로그인

CV 열람하기

연락처 보기