박대훈 (Yale University School of Medicine, 가톨릭대학교 의생명과학과) | 한빛사논문 > 한빛사

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조회516

Yale University School of Medicine, 가톨릭대학교 의생명과학과

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Proc. Natl. Acad. Sci. USA, Jul 16 2024, 121 (29) | https://doi.org/10.1073/pnas.2409605121 Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles

Daehun Park^a,b,c,d,e, Kenshiro Fujise^a,b,c, Yumei Wu^a,b,c, Rafael Luján^f, Sergio Del Olmo-Cabrera^g, John F. Wesseling^g, and Pietro De Camilli^a,b,c,1

^aDepartment of Neuroscience, Yale University School of Medicine, New Haven, CT 06510;

^bDepartment of Cell biology, Yale University School of Medicine, New Haven, CT 06510;

^cProgram in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510;

^dDepartment of Medical and Biological Sciences, The Catholic University of Korea, Bucheon 14662, South Korea;

^eDepartment of Biotechnology, The Catholic University of Korea, Bucheon 14662, South Korea;

^fSynaptic Structure Laboratory, Departamento de Ciencias Médicas, Instituto de Biomedicina de la Universidad de Castilla-La Mancha, Facultad de Medicina, University of Castilla-La Mancha, Albacete 02006, Spain;

^gInstitute for Neurosciences Consejo Superior de Investigaciones Científicas-Universidad Miguel Hernández, San Juan de Alicante 03550, Spain

¹To whom correspondence may be addressed. : Pietro De Camilli

Abstract

Members of the synaptophysin and synaptogyrin family are vesicle proteins with four transmembrane domains. In spite of their abundance in synaptic vesicle (SV) membranes, their role remains elusive and only mild defects at the cellular and organismal level are observed in mice lacking one or more family members. Here, we show that coexpression with synapsin in fibroblasts of each of the four brain-enriched members of this family-synaptophysin, synaptoporin, synaptogyrin 1, and synaptogyrin 3-is sufficient to generate clusters of small vesicles in the same size range of SVs. Moreover, mice lacking all these four proteins have larger SVs. We conclude that synaptophysin and synaptogyrin family proteins play an overlapping function in the biogenesis of SVs and in determining their small size.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-11)
연구진국내+국외 연구진
분야 생명과학 > 신경생물학

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