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Biosens. Bioelectron., Jul 06 2024, 262 S0956-5663(24)00564-5 | https://doi.org/10.1016/j.bios.2024.116559
Label-free detection of glutathione and glutathione disulfide in biological fluid by using an alpha-hederin nanopore
Sang-Mook You 1,2, Ki-Baek Jeong 1,3, Dong-Gook Kang, Sang-Min Kim, Young-Rok Kim
Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104, Republic of Korea
1S.M.Y. and K.B.J contributed equally to this work.
2Present address: Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44429, Republic of Korea.
3Present address: Disease Target Structure Research Center, Division of Biomedical Research, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Corresponding author : Young-Rok Kim
Abstract
Glutathione (GSH) is indispensable for maintaining redox homeostasis in biological fluids and serves as a key component in cellular defense mechanisms. Accurate assessment of GSH relative to its oxidized counterpart, glutathione disulfide (GSSG), is critical for the early diagnosis and understanding of conditions related to oxidative stress. Despite existing methods for their quantification, the label-free and simultaneous measurement of GSH and GSSG in biological fluid presents significant challenges. Herein, we report the use of an alpha-hederin (Ah) nanopore for the direct measurement of the GSH:GSSG ratio in simulated biological fluid, containing fetal bovine serum (FBS). This system hinges on detecting characteristic relative ion blockades (ΔI/I
o) as GSH and GSSG molecules pass through the Ah nanopore under an applied electric field. The distinct current blockage signals derived from the translocation of GSH and GSSG enabled us to determine the molar ratio of GSH and its oxidized form. Notably, the interactions between the hydroxyl groups of the sugar moiety lining the nanopore's inner surface and the sulfhydryl group of GSH significantly influence the translocation dynamics, resulting in a longer translocation time for GSH compared to GSSG. The Ah nanopore technology proposed in this study offers a promising approach for real-time, single molecule-level monitoring of glutathione redox status in biological fluids, eliminating the need for labeling or extensive sample preparation.
논문정보
- Research article
- 2024년 07월 (BRIC 등록일 2024-07-10)
- 국내 연구진
- 생명과학 > 식품학
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