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- 조회445
Nano Lett., Jul 08 2024, | https://doi.org/10.1021/acs.nanolett.4c00447
iMAX FRET (Information Maximized FRET) for Multipoint Single-Molecule Structural Analysis
Bhagyashree S. Joshi 1, Carlos de Lannoy 1, Mark R. Howarth 2, Sung Hyun Kim 1,3,4, Chirlmin Joo 1,3
1Kavli Institute of Nanoscience, Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, The Netherlands.
2Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
3Department of Physics, Ewha Womans University, Seoul 03760, Republic of Korea.
4New and Renewable Energy Research Center, Ewha Womans University, Seoul 03760, Republic of Korea.
Corresponding Authors : Sung Hyun Kim, Chirlmin Joo
Abstract
Understanding the structure of biomolecules is vital for deciphering their roles in biological systems. Single-molecule techniques have emerged as alternatives to conventional ensemble structure analysis methods for uncovering new biology in molecular dynamics and interaction studies, yet only limited structural information could be obtained experimentally. Here, we address this challenge by introducing
iMAX FRET, a one-pot method that allows
ab initio 3D profiling of individual molecules using two-color FRET measurements. Through the stochastic exchange of fluorescent weak binders,
iMAX FRET simultaneously assesses multiple distances on a biomolecule within a few minutes, which can then be used to reconstruct the coordinates of up to four points in each molecule, allowing structure-based inference. We demonstrate the 3D reconstruction of DNA nanostructures, protein quaternary structures, and conformational changes in proteins. With
iMAX FRET, we provide a powerful approach to advance the understanding of biomolecular structure by expanding conventional FRET analysis to three dimensions.
논문정보
- Research article
- 2024년 07월 (BRIC 등록일 2024-07-10)
- 국내(교신)+국외 연구진
- 생명과학 > 구조생물 및 생물물리학
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