이병택 (순천향대학교)

한빛사논문

조회89

순천향대학교

Biomaterials, Jul 02 2024, 311 S0142-9612(24)00232-1 | https://doi.org/10.1016/j.biomaterials.2024.122698 ECM derivatized alginate augmenting bio-functionalities of lyophilized mat for skin and liver wound treatment

Md Sohanur Rahaman ^a, Asuva Arin ^a, Ume Farwa ^c, Myeongki Park ^a, Sang Ho Bae^b, Byong-Taek Lee ^a,c

^aDepartment of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, 31151, Republic of Korea

^bDepartment of Surgery, Soonchunhyang University Cheonan Hospital, Cheonan 31151, Republic of Korea

^cInstitute of Tissue Regeneration, Soonchunhyang University, Cheonan-31151, Republic of Korea

Corresponding author : Byong-Taek Lee

Abstract

Peptides and molecular residues sourced from the fragmentation of the extracellular matrix (ECM) can exacerbate a plethora of cellular functions. We selected a natural ECM-derived complex peptide mixture to functionalize sodium alginate. Three alginate derivatives (sodium alginate conjugated with ECM) SALE-1, SALE-2, and SALE-3 were synthesized using the lowest (10 % w/w), moderate (50 % w/w), and highest (100 % w/w) concentrations of ECM. Thereafter, they were used to fabricate three groups of mat scaffolds EMAT-1 (ECM derivatized alginate thrombin-mat), EMAT-2, and EMAT-3, respectively by the freeze-drying process. To enhance the hemostatic activity, thrombin was loaded onto the scaffolds. Another group, AT, without any derivatized alginate was additionally included in order to comparative analysis. Physical characteristics revealed that the porous mat scaffold showed enhancement in degradation and swelling ability with the increase in ECM content. The higher cell proliferation, migration, and cell viability were noticed in the higher ECM-containing samples EMAT-2 and EMAT-3. In vivo studies using rodent hepatic and rabbit ear models were carried out to ensure the hemostatic ability of the scaffolds. EMAT-2 and EMAT-3 demonstrate excellent liver regeneration ability in rat models. Moreover, the rat cutaneous wound model depicted that EMAT-3 dramatically elevated the skin's healing ability, thereby rendering it an excellent candidate for future clinical application in wound healing.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-10)
연구진국내 연구진
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