최서현 (연세대학교 IBS 나노의학연구단)

한빛사논문

조회550

연세대학교 IBS 나노의학연구단

CV File 316 kb

Nat. Nanotechnol., Jul 02 2024, | https://doi.org/10.1038/s41565-024-01694-2 In vivo magnetogenetics for cell-type-specific targeting and modulation of brain circuits

Seo-Hyun Choi¹, Jihye Shin^1,2, Chanhyun Park¹, Jung-uk Lee¹, Jaegyeong Lee¹, Yuko Ambo¹, Wookjin Shin¹, Ri Yu¹, Ju-Young Kim^1,2, Jungsu David Lah^1,2, Donghun Shin^1,2, Gooreum Kim^1,3, Kunwoo Noh^1,2, Wuhyun Koh^4,5, C. Justin Lee^4,5, Jae-Hyun Lee^1,2, Minsuk Kwak^1,2 & Jinwoo Cheon^1,2,3

¹Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea.

²Department of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, Republic of Korea.

³Department of Chemistry, Yonsei University, Seoul, Republic of Korea.

⁴IBS School, University of Science and Technology (UST), Daejeon, Republic of Korea.

⁵Center for Cognition and Sociality, Life Science Cluster, Institute for Basic Science (IBS), Daejeon, Republic of Korea.

Corresponding authors

Correspondence to Jae-Hyun Lee, Minsuk Kwak or Jinwoo Cheon.

Abstract

Neuromodulation technologies are crucial for investigating neuronal connectivity and brain function. Magnetic neuromodulation offers wireless and remote deep brain stimulations that are lacking in optogenetic- and wired-electrode-based tools. However, due to the limited understanding of working principles and poorly designed magnetic operating systems, earlier magnetic approaches have yet to be utilized. Furthermore, despite its importance in neuroscience research, cell-type-specific magnetic neuromodulation has remained elusive. Here we present a nanomaterials-based magnetogenetic toolbox, in conjunction with Cre-loxP technology, to selectively activate genetically encoded Piezo1 ion channels in targeted neuronal populations via torque generated by the nanomagnetic actuators in vitro and in vivo. We demonstrate this cell-type-targeting magnetic approach for remote and spatiotemporal precise control of deep brain neural activity in multiple behavioural models, such as bidirectional feeding control, long-term neuromodulation for weight control in obese mice and wireless modulation of social behaviours in multiple mice in the same physical space. Our study demonstrates the potential of cell-type-specific magnetogenetics as an effective and reliable research tool for life sciences, especially in wireless, long-term and freely behaving animals.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-04)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 다학제 융합/복합

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