Roniya Thapa Magar (동아대학교)

한빛사논문

조회157

Roniya Thapa MagarRoniya Thapa Magar

동아대학교

ISME J., Jul 02 2024, wrae120 | https://doi.org/10.1093/ismejo/wrae120 Rhizobacterial syntrophy between a helper and a beneficiary promotes tomato plant health

Sang-Moo Lee^a,Ϯ, Roniya Thapa Magar^b,Ϯ, Min Kyeong Jung^b,Ϯ, Hyun Gi Kong^b,c, Ju Yeon Song^d, Joo Hwan Kwon^b, Minseo Choi^b, Hyoung Ju Lee^b, Seung Yeup Lee^b, Raees Khan^b,e, Jihyun F. Kim^d,f and Seon-Woo Lee^a,b*

^aInstitute of Agricultural Life Sciences, Dong-A University, Busan 49315, Republic of Korea

^bDepartment of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea

^cDepartment of Plant Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea

^dDepartment of Systems Biology and Institute for Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea

^eDepartment of Sciences, National University of Medical Sciences, Rawalpindi, 46000, Pakistan

^fMicrobiome Initiative, Yonsei University, Seoul 03722, Republic of Korea

^ϮThese authors contributed equally to this work.

^*Address correspondence to Seon-Woo Lee

Abstract

Microbial interactions impact the functioning of microbial communities. However, microbial interactions within host-associated communities remains poorly understood. Here, we report that the beneficiary rhizobacterium Niallia sp. RD1 requires the helper Pseudomonas putida H3 for bacterial growth and beneficial interactions with the plant host. In the absence of the helper H3 strain, the Niallia sp. RD1 strain exhibited weak respiration and elongated cell morphology without forming bacterial colonies. A transposon mutant of H3 in a gene encoding succinate-semialdehyde dehydrogenase displayed much attenuated support of RD1 colony formation. Through subsequent addition of succinate to the media, we found that succinate serves as a public good that supports RD1 growth. Comparative genome analysis highlighted that RD1 lacked the gene for sufficient succinate, suggesting its evolution as a beneficiary of succinate biosynthesis. The syntrophic interaction between RD1 and H3 efficiently protected tomato plants from bacterial wilt and promoted the tomato growth. The addition of succinate to the medium restored complex II-dependent respiration in RD1 and facilitated the cultivation of various bacterial isolates from the rhizosphere. Taken together, we delineate energy auxotrophic beneficiaries ubiquitous in the microbial community, and these beneficiaries could benefit host plants with the aid of helpers in the rhizosphere.

논문정보

형식Research article
게재일2024년 07월 (BRIC 등록일 2024-07-04)
연구진국내(교신)+국외 연구진
분야 생명과학 > 식량작물 및 원예작물

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