한빛사논문
- 조회150
Kyung Kwan Lee a, Jae Yoon Shin b, Sang Cheon Lee c, Chang-Soo Lee a,d
aBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Daejeon 34141, Republic of Korea
bDepartment of Advanced Materials Chemistry, Korea University, Sejong 30019, Republic of Korea
cDepartment of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
dDepartment of Biotechnology, University of Science & Technology (UST), Daejeon 34113, Republic of Korea
Corresponding author : Chang-Soo Lee
Abstract
Fluorescent polydopamine (PDA) nanoparticles (FPNPs) have gained recognition as invaluable resources for a wide range of biomedical applications. Typically, these FPNPs are synthesized solely from dopamine (DA). In this study, our focus lies in the design of PDA-based multicolor fluorescent nanoparticles (m-FNPs) utilizing DA and its analogues, including levodopa (LVD), norepinephrine (NPP), 6-hydroxydopamine (HDA), and epinephrine (EPP) monomers, thereby paving the way for the next generation of FPNPs. The m-FNPs, respectively labeled as L-FNPs, N-FNPs, H-FNPs, E-FNPs, and D-FNPs, exhibit distinct band gaps influenced by the monomer’s structure, allowing versatile multicolor fluorescence bioimaging across a broad fluorescence emission spectrum ranging from 410 to 680 nm. Significantly, the catechol groups present on the surfaces of m-FNPs enable chelation with various theranostic metal ions and subsequent release under endo/lysosome pH conditions in cancer cells, thereby eliciting a cancer-specific “OFF-ON” fluorescence signal in vivo. Furthermore, integrating m-FNPs into poly(dimethylsiloxane) (PDMS) produces a tissue-adhesive fluorescence sheet that permits fluorescence monitoring of internal tissue surfaces within living organisms for 15 days. These m-FNPs-incorporated PDMS (m-FNPs-PDMS) sheets, enriched with catechol, carboxyl, and amine groups on their surfaces, exhibit significantly enhanced adhesion to biological tissues compared to conventional PDMS sheets containing PDA. Our findings suggest that m-FNPs pave the way for advancing PDA-based fluorescent nanoparticles with diverse applications in the biomedical field, supported by comprehensive in vitro and in vivo evaluations.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-07-01)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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