김원종 (POSTECH)

한빛사논문

조회136

POSTECH

CV File 773 kb

ACS Nano, Jun 28 2024, | https://doi.org/10.1021/acsnano.3c12551 3D Multiparametric Photoacoustic Computed Tomography of Primary and Metastatic Tumors in Living Mice

Jiwoong Kim ¹, Jihye Lee², Seongwook Choi ¹, Hyori Lee ², Jinge Yang¹, Hyunseo Jeon ¹, Minsik Sung ¹, Won Jong Kim ², Chulhong Kim¹

¹Department of Electrical Engineering, Convergence IT Engineering, Mechanical Engineering, and Medical Science and Engineering, Medical Device Innovation Center, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.

²Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.

J.K., J.L., and S.C. contributed equally to this work.

Corresponding Authors : Won Jong Kim, Chulhong Kim

Abstract

Photoacoustic computed tomography (PACT), an emerging imaging modality in preclinical cancer research, can provide multiparametric 3D information about structures, physiological functions, and pharmacokinetics. Here, we demonstrate the use of high-definition 3D multiparametric PACT imaging of both primary and metastatic tumors in living mice to noninvasively monitor angiogenesis, carcinogenesis, hypoxia, and pharmacokinetics. The high-definition PACT system with a 1024-element hemispherical ultrasound transducer array provides an isotropic spatial resolution of 380 μm, an effective volumetric field-of-view of 12.8 mm × 12.8 mm × 12.8 mm without scanning, and an acquisition time of <30 s for a whole mouse body. Initially, we monitor the structural progression of the tumor microenvironment (e.g., angiogenesis and vessel tortuosity) after tumor cell inoculation. Then, we analyze the change in oxygen saturation of the tumor during carcinogenesis, verifying induced hypoxia in the tumor's core region. Finally, the whole-body pharmacokinetics are photoacoustically imaged after intravenous injection of micelle-loaded IR780 dye, and the in vivo PACT results are validated in vivo and ex vivo by fluorescence imaging. By employing the premium PACT system and applying multiparametric analyses to subcutaneous primary tumors and metastatic liver tumors, we demonstrate that this PACT system can provide multiparametric analyses for comprehensive cancer research.

논문정보

형식Research article
게재일2024년 06월 (BRIC 등록일 2024-06-30)
연구진국내 연구진
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