한빛사논문
- 조회283
한양대학교
Mingyu Shin 1,6, Eunji Chang 1,6, Daewon Lee 1,6, Nayun Kim 2, Bumsik Cho 1, Nuri Cha 1, Ferdinand Koranteng 1, Ji-Joon Song 2 & Jiwon Shim 1,3,4,5,*
1Department of Life Science, College of Natural Science, Hanyang University, Seoul, Republic of Korea.
2Department of Biological Sciences, KI for BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
3Research Institute for Natural Science, Hanyang University, Seoul, Republic of Korea.
4Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, Republic of Korea.
5Hanyang Institute of Advanced BioConvergence, Hanyang University, Seoul, Republic of Korea.
6These authors contributed equally: Mingyu Shin, Eunji Chang, Daewon Lee
*Corresponding author: correspondence to Jiwon Shim
Abstract
Insect respiration has long been thought to be solely dependent on an elaborate tracheal system without assistance from the circulatory system or immune cells. Here we describe that Drosophila crystal cells—myeloid-like immune cells called haemocytes—control respiration by oxygenating Prophenoloxidase 2 (PPO2) proteins. Crystal cells direct the movement of haemocytes between the trachea of the larval body wall and the circulation to collect oxygen. Aided by copper and a neutral pH, oxygen is trapped in the crystalline structures of PPO2 in crystal cells. Conversely, PPO2 crystals can be dissolved when carbonic anhydrase lowers the intracellular pH and then reassembled into crystals in cellulo by adhering to the trachea. Physiologically, larvae lacking crystal cells or PPO2, or those expressing a copper-binding mutant of PPO2, display hypoxic responses under normoxic conditions and are susceptible to hypoxia. These hypoxic phenotypes can be rescued by hyperoxia, expression of arthropod haemocyanin or prevention of larval burrowing activity to expose their respiratory organs. Thus, we propose that insect immune cells collaborate with the tracheal system to reserve and transport oxygen through the phase transition of PPO2 crystals, facilitating internal oxygen homeostasis in a process that is comparable to vertebrate respiration.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-06-27)
- 국내 연구진
- 생명과학 > 발생생물학
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