박영준 (경희대학교)

한빛사논문

조회252

박영준Young-Joon Park

경희대학교

CV File 314 kb

Hortic. Res.-England, Jun 2024, 11 (6) uhae126 | https://doi.org/10.1093/hr/uhae126 Evolutionary conservation of receptor compensation for stem cell homeostasis in Solanaceae plants

Myeong-Gyun Seo^1,‡, Yoonseo Lim^1,‡, Anat Hendelman^2,3, Gina Robitaille^2,3, Hong Kwan Beak⁴, Woo-Jong Hong⁵, Soon Ju Park⁶, Zachary B. Lippman^2,3, Young-Joon Park^5,*, and Choon-Tak Kwon^1,5,*

¹Graduate School of Green-Bio Science, Kyung Hee University, Yongin 17104, Republic of Korea

²Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

³Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA

⁴Division of Biological Sciences and Research Institute for Basic Science, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea

⁵Department of Smart Farm Science, Kyung Hee University, Yongin 17104, Republic of Korea.

⁶Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Republic of Korea

^*Corresponding authors: Young-Joon Park, Choon-Tak Kwon

^‡These authors contributed equally to this work.

Abstract

Stem cell homeostasis is pivotal for continuous and programmed formation of organs in plants. The precise control of meristem proliferation is mediated by the evolutionarily conserved signaling that encompasses complex interactions among multiple peptide ligands and their receptor-like kinases. Here, we identified compensation mechanisms involving the CLAVATA1 (CLV1) receptor and its paralogs, BARELY ANY MERISTEMs (BAMs), for stem cell proliferation in two Solanaceae species, tomato and groundcherry. Genetic analyses of higher-order mutants deficient in multiple receptor genes, generated via CRISPR-Cas9 genome editing, reveal that tomato SlBAM1 and SlBAM2 compensate for slclv1 mutations. Unlike the compensatory responses between orthologous receptors observed in Arabidopsis, tomato slclv1 mutations do not trigger transcriptional upregulation of four SlBAM genes. The compensation mechanisms within receptors are also conserved in groundcherry, and critical amino acid residues of the receptors associated with the physical interaction with peptide ligands are highly conserved in Solanaceae plants. Our findings demonstrate that the evolutionary conservation of both compensation mechanisms and critical coding sequences between receptor-like kinases provides a strong buffering capacity during stem cell homeostasis in tomato and groundcherry.

논문정보

형식Research article
게재일2024년 06월 (BRIC 등록일 2024-06-27)
연구진국내 연구진
분야 생명과학 > 식량작물 및 원예작물

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