한빛사논문
- 조회935
Szu-Hsien Sam Wu 1,2, Somi Kim 3, Heetak Lee 4, Ji-Hyun Lee 4, So-Yeon Park 4, Réka Bakonyi 1, Isaree Teriyapirom 1,2, Natalia Hallay 1, Sandra Pilat-Carotta 1, Hans-Christian Theussl 5, Jihoon Kim 4,6, Joo-Hyeon Lee 7,8, Benjamin D. Simons 7,9,10, Jong Kyoung Kim 3, Gabriele Colozza 1,* & Bon-Kyoung Koo 3,4,*
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
2Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna, Vienna, Austria.
3Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
4Center for Genome Engineering, Institute for Basic Science, Expo-ro 55, Yuseong-gu, Daejeon 34126, Republic of Korea.
5IMP/IMBA Transgenic Service, Institute of Molecular Pathology (IMP), Vienna, Austria.
6Department of Medical and Biological Sciences, The Catholic University of Korea, Bucheon 14662, South Korea.
7Wellcome–MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
8Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
9The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
10Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, Wilberforce Road, Cambridge CB3 0WA, UK.
*Corresponding authors: correspondence to Gabriele Colozza or Bon-Kyoung Koo
Abstract
Image-based lineage tracing enables tissue turnover kinetics and lineage potentials of different adult cell populations to be investigated. Previously, we reported a genetic mouse model system, Red2Onco, which ectopically expressed mutated oncogenes together with red fluorescent proteins (RFP). This system enabled the expansion kinetics and neighboring effects of oncogenic clones to be dissected. We now report Red2Flpe-SCON: a mosaic knockout system that uses multicolor reporters to label both mutant and wild-type cells. We develop the Red2Flpe mouse line for red clone-specific Flpe expression, as well as the FRT-based SCON (Short Conditional IntrON) method to facilitate tunable conditional mosaic knockouts in mice. We use the Red2Flpe-SCON method to study Sox2 mutant clonal analysis in the esophageal epithelium of adult mice which reveal that the stem cell gene, Sox2, is less essential for adult stem cell maintenance itself, but rather for stem cell proliferation and differentiation.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-06-13)
- 국내(교신)+국외 연구진
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