조성우 (연세대학교 의과대학)

한빛사논문

조회240

조성우Sungwoo Cho

연세대학교 의과대학

Small, Jun 10 2024, | https://doi.org/10.1002/smll.202401989 Surface Crystal and Degradability of Shape Memory Scaffold Essentialize Osteochondral Regeneration

Sungwoo Cho ¹, Kang Suk Lee ^1,2, Kyubae Lee ³, Hye-Seon Kim⁴, Suji Park ¹, Seung Eun Yu ¹, Hyunsu Ha ¹, Sewoom Baek ^1,5, Jueun Kim ^1,5, Hyunjae Kim ¹, Ji Youn Lee ⁵, Sangmin Lee ¹, Hak-Joon Sung^1,2,5

¹Department of Medical Engineering, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

²TMD LAB Co. Ltd., 6th Floor, 31, Gwangnaru-ro 8-gil, Seongdong-gu, Seoul, 04799, South Korea.

³Department of Biomedical Materials, Konyang University, 158, Gwanjeodong-ro, Seo-gu, Daejeon, 35365, South Korea.

⁴Department of Anesthesiology, Perioperative, and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.

⁵Department of Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

S.C. and K.S.L. contributed equally to this work as co-ﬁrst authors.

CORRESPONDING AUTHOR : Hak-Joon Sung

Abstract

The minimally invasive deployment of scaffolds is a key safety factor for the regeneration of cartilage and bone defects. Osteogenesis relies primarily on cell-matrix interactions, whereas chondrogenesis relies on cell-cell aggregation. Bone matrix expansion requires osteoconductive scaffold degradation. However, chondrogenic cell aggregation is promoted on the repellent scaffold surface, and minimal scaffold degradation supports the avascular nature of cartilage regeneration. Here, a material satisfying these requirements for osteochondral regeneration is developed by integrating osteoconductive hydroxyapatite (HAp) with a chondroconductive shape memory polymer (SMP). The shape memory function-derived fixity and recovery of the scaffold enabled minimally invasive deployment and expansion to fill irregular defects. The crystalline phases on the SMP surface inhibited cell aggregation by suppressing water penetration and subsequent protein adsorption. However, HAp conjugation SMP (H-SMP) enhanced surface roughness and consequent cell-matrix interactions by limiting cell aggregation using crystal peaks. After mouse subcutaneous implantation, hydrolytic H-SMP accelerated scaffold degradation compared to that by the minimal degradation observed for SMP alone for two months. H-SMP and SMP are found to promote osteogenesis and chondrogenesis, respectively, in vitro and in vivo, including the regeneration of rat osteochondral defects using the binary scaffold form, suggesting that this material is promising for osteochondral regeneration.

논문정보

형식Research article
게재일2024년 06월 (BRIC 등록일 2024-06-11)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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