한빛사
>
한빛사논문
한빛사논문
- 조회301
Bioact. Mater., Available online 9 May 2024 | https://doi.org/10.1016/j.bioactmat.2024.05.011
Ultrasound-triggered three dimensional hyaluronic acid hydrogel promotes in vitro and in vivo reprogramming into induced pluripotent stem cells
Deogil Kim 1, Min-Ju Lee 1, Yoshie Arai, Jinsung Ahn, Gun Woo Lee, Soo-Hong Lee
Department of Biomedical Engineering, Dongguk University-Seoul, 04620, Seoul, South Korea
1These authors contributed equally to this work.
Corresponding author : Soo-Hong Lee
Abstract
Cellular reprogramming technologies have been developed with different physicochemical factors to improve the reprogramming efficiencies of induced pluripotent stem cells (iPSCs). Ultrasound is a clinically applied noncontact biophysical factor known for regulating various cellular behaviors but remains uninvestigated for cellular reprogramming. Here, we present a new reprogramming strategy using low-intensity ultrasound (LIUS) to improve cellular reprogramming of iPSCs in vitro and in vivo. Under 3D microenvironment conditions, increased LIUS stimulation shows enhanced cellular reprogramming of the iPSCs. The cellular reprogramming process facilitated by LIUS is accompanied by increased mesenchymal to epithelial transition and histone modification. LIUS stimulation transiently modulates the cytoskeletal rearrangement, along with increased membrane fluidity and mobility to increase HA/CD44 interactions. Furthermore, LIUS stimulation with HA hydrogel can be utilized in application of both human cells and in vivo environment, for enhanced reprogrammed cells into iPSCs. Thus, LIUS stimulation with a combinatorial 3D microenvironment system can improve cellular reprogramming in vitro and in vivo environments, which can be applied in various biomedical fields.
논문정보
- Research article
- 2024년 05월 (BRIC 등록일 2024-06-10)
- 국내 연구진
- 바이오·의료융합 > 다학제 융합/복합
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
