한빛사논문
- 조회200
Wanho Cho a,1, Jinhee Park a,1, Wijin Kim a,1, Wei Mao a,d, Jongmin Park b,d, Young Mee Jung b,c,d, Ju Hyun Park a,d, Hyuk Sang Yoo a,c,d,e
aDepartment of Biomedical Science, Kangwon National University, Chuncheon-si, Gangwon-do 24341, Republic of Korea
bDepartment of Chemistry, Kangwon National University, Chuncheon-si, Gangwon-do 24341, Republic of Korea
cKangwon Radiation Convergence Research Center, Kangwon National University, Chuncheon, 24341, Republic of Korea
dInstitute for Molecular Science and Fusion Technology, Kangwon National University, Chuncheon 24341, Republic of Korea
eInstitute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Republic of Korea
1These authors contributed equally.
Corresponding authors : Ju Hyun Park, Hyuk Sang Yoo
Abstract
The spheroid formation derived from human-induced pluripotent stem cells (hiPSCs) generates a three-dimensional (3D) microenvironment that fosters cell–cell interactions, modulates epigenetic regulation, and imitates early embryonic development, all of which contribute to biomedical applications including drug discovery, disease modeling, and cell replacement therapy. In this study, we developed 3D hiPSC spheroids by incorporating surface-engineered nanofibrils to enhance their pluripotency and differentiation capability. Polymeric nanofibrils were chemically immobilized with cell adhesion peptides that mimic the properties of vitronectin and laminin. The incorporation of nanofibrils into hiPSC spheroids significantly improved their viability and undifferentiated state, which may be due to the facilitated mass transport across the spheroids, enabled by the loosely held structure of cells and nanofibrils. By harnessing the enhanced pluripotency of hiPSC spheroids with nanofibrils, we induced differentiation into the hepatic lineage and found a significant upregulation of mature hepatocyte-specific markers in spheroids with nanofibrils compared with those without nanofibrils. Therefore, the combination of 3D hiPSC spheroids with surface-engineered nanofibrils offers a promising approach to enhancing pluripotency and hepatic differentiation, with potential implications for regenerative medicine.
논문정보
- Research article
- 2024년 05월 (BRIC 등록일 2024-06-10)
- 국내 연구진
- 바이오·의료융합 > 바이오소재
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