한빛사논문
- 조회243
연세대학교 의과대학
Mirae Lee 1,2, Jiwon Woo 1, Kyoung-Tae Kim 3, Seul-A Moon 3, Hyeong Cheon Park 4, Tae Yeon Kim 4, Jeong-Yoon Park 1,2
1The Spine and Spinal Cord Institute, Department of Neurosurgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, 06273, Republic of Korea.
2Department of Neurosurgery, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.
3Department of Neurosurgery, Kyungpook National University Hospital, Daegu, 41944, Republic of Korea.
4Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, 06273, Republic of Korea.
M.L. and J.W. contributed equally to this work.
CORRESPONDING AUTHOR : Jeong-Yoon Park
Abstract
Tissue biopsy for early diagnosis and monitoring comes with several challenges, such as its invasiveness, and issues related to tissue heterogeneity in sampling. To address these issues, researchers have proposed a non-invasive approach called liquid biopsy, which uses blood samples to detect specific non-coding RNA (miRNA). However, the current process of isolating and amplifying miRNA can be time-consuming and yield non-specific results. In this study, we introduce a new super-resolution imaging tool that utilizes a thin, hydrogel-based liquid view (LV) film. This film can undergo a 9-fold expansion and allows the analysis of cells obtained from liquid biopsy. We have validated the potential of the LV film as a tool for early diagnosis and prognosis by testing biofluids derived from a variety of diseases. This method has been confirmed to accurately analyze a greater number of miRNAs with higher sensitivity in a shorter time compared to other analytical methods. Our findings suggest that the LV film provides high specificity, and multiplexing in detecting small amounts of miRNAs within cells, making it suitable for three-dimensional implementation. We propose that liquid biopsy with LV films could be a solution to limitations related to the invasiveness, cost, and time-consuming nature of molecular analysis.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-06-18)
- 국내 연구진
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