한빛사논문
- 조회396
Siwoo Jin 1, Yongdeok Ahn 1, Jiseong Park 1, Minsoo Park 1, Sang-Chul Lee 2, Wonhee J Lee 1 *, Daeha Seo 1 *
1Department of Physics and Chemistry, DGIST, Daegu, 42988, Republic of Korea.
2Division of Nanotechnology, and Department of DGIST, Daegu, 42988, Republic of Korea.
*Corresponding authors: correspondence to Wonhee J Lee or Daeha Seo
Abstract
The material transport system, facilitated by motor proteins, plays a vital role in maintaining a non-equilibrium cellular state. However, understanding the temporal coordination of motor protein activity requires an advanced imaging technique capable of measuring 3D angular displacement in real-time. In this study, a Fourier transform-based plasmonic dark-field microscope has been developed using anisotropic nanoparticles, enabling the prolonged and simultaneous observation of endosomal lateral and rotational motion. A sequence of discontinuous 3D angular displacements has been observed during the pause and run phases of transport. Notably, a serially correlated temporal pattern in the intermittent rotational events has been demonstrated during the tug-of-war mechanism, indicating Markovian switching between the exploitational and explorational modes of motor protein exchange prior to resuming movement. Alterations in transition frequency and the exploitation-to-exploration ratio upon dynein inhibitor treatment highlight the relationship between disrupted motor coordination and reduced endosomal transport efficiency. Collectively, these results suggest the importance of orchestrated temporal motor protein patterns for efficient cellular transport.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-06-04)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
관련 보도자료
- Bio통신원
- 2024. 07. 16
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