한빛사논문
- 조회248
Wonhyoung Park a,1, Hyewon Jang b,1, Hee Seung Kim c,d, Soo Jin Park c, Whasun Lim b, Gwonhwa Song a, Sunwoo Park e,f
aInstitute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea
bDepartment of Biological Sciences, College of Science, Sungkyunkwan University, Suwon, 16419, Republic of Korea
cDepartment of Obstetrics and Gynecology, Seoul National University Hospital, Seoul 03080, Republic of Korea
dDepartment of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea
eDepartment of Plant & Biomaterials Science, Gyeongsang National University, Jinju, 52725, Republic of Korea
fDepartment of GreenBio Science, Gyeongsang National University, Jinju, 52725, Republic of Korea
1These authors contributed equally to this work.
Corresponding authors: Whasun Lim, Gwonhwa Song, Sunwoo Park
Abstract
Background: Baicalein is a flavonoid extracted from the roots of Scutellaria baicalensis G. that has anti-inflammatory and antitumor effects. However, therapeutic mechanisms of baicalein in patients with endometriosis in vivo have yet to be elucidated. As a chronic inflammatory gynecological disease, endometriosis causes pain and infertility, and has no complete treatment to date. Current treatment strategies cause several side effects and have high recurrence rates.
Purpose: This study aimed to identify the in vivo therapeutic effects of baicalein on endometriosis and verify the action mechanisms of baicalein, focusing on regulating inflammation.
Methods: In this study, an autologous transplant mouse model and patient-derived immortalized human ovarian endometriotic stromal cells (ihOESCs) were used to investigate the therapeutic activities of baicalein. The mouse model was administered with 40 mg/kg baicalein by oral gavage for 4 weeks, and the treatment outcomes of baicalein-treated mice were compared with vehicle- and dienogest-treated groups. ihOESCs were treated with 0-5 μg/ml baicalein for in vitro studies.
Results: Baicalein significantly alleviated the progression of endometriosis in mouse models. Baicalein reduced the expression of proinflammatory cytokines in endometriotic lesions and ihOESCs, and cytokine expression and T cell proportions in mouse spleen. in vitro results showed that baicalein increased mitochondrial calcium flux and induced mitochondrial depolarization and ROS generation in ihOESCs. Ultimately, baicalein inactivated the MAPK/PI3K signaling and induced cell death in ihOESCs.
Conclusion: In conclusion, baicalein effectively attenuated the progression of endometriosis through its anti-inflammatory activities. Baicalein can be an alternative or supplemental treatment for endometriosis to ameliorate the side effects of hormonal therapy.
논문정보
- Research article
- 2024년 03월 (BRIC 등록일 2024-06-03)
- 국내 연구진
- 생명과학 > 발생생물학
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