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Small, Jun 02 2024, | https://doi.org/10.1002/smll.202401629 Energy Transfer Between i-Motif DNA Encapsulated Silver Nanoclusters and Fluorescein Amidite Efficiently Visualizes the Redox State of Live Cells

Hari Chandana Yadavalli ¹, Yeolhoe Kim ¹, Il Lae Jung ², Sooyeon Park ¹, Tae-Hwan Kim ³, Jin Young Shin ⁴, Riddhi Nagda ¹, Peter Waaben Thulstrup ⁵, Morten Jannik Bjerrum ⁵, Yong Joo Bhang ⁶, Phil Hyu Lee⁴, Won Ho Yang¹, Pratik Shah ⁷, Seong Wook Yang ¹

¹Department of Systems Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul, 03722, Republic of Korea.

²Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon, 34057, Republic of Korea.

³Department of Quantum System Engineering, Jeonbuk National University, Jeonju, 54896, Republic of Korea.

⁴Department of Neurology, College of Medicine, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.

⁵Department of Chemistry, University of Copenhagen, Universitetsparken 5, Copenhagen, 2100, Denmark.

⁶Xenohelix Research Institute, BT Centre 305, 56 Songdogwahak-ro Yeonsugu, Incheon, 21984, Republic of Korea.

⁷Department of Science and Environment, Roskilde University, Roskilde, 4000, Denmark.

H.C.Y., Y.K., and I.L.J. contributed equally to this work.

CORRESPONDING AUTHORS: Won Ho Yang, Pratik Shah, Seong Wook Yang

Abstract

The redox regulation, maintaining a balance between oxidation and reduction in living cells, is vital for cellular homeostasis, intricate signaling networks, and appropriate responses to physiological and environmental cues. Here, a novel redox sensor, based on DNA-encapsulated silver nanoclusters (DNA/AgNCs) and well-defined chemical fluorophores, effectively illustrating cellular redox states in live cells is introduced. Among various i-motif DNAs, the photophysical property of poly-cytosines (C ₂₀)-encapsulated AgNCs that sense reactive oxygen species (ROS) is adopted. However, the sensitivity of C ₂₀/AgNCs is insufficient for evaluating ROS levels in live cells. To overcome this drawback, the ROS sensing mechanism of C ₂₀/AgNCs through gel electrophoresis, mass spectrometry, and small-angle X-ray scattering is primarily defined. Then, by tethering fluorescein amidite (FAM) and Cyanine 5 (Cy5) dyes to each end of the C ₂₀/AgNCs sensor, an Energy Transfer (ET) between AgNCs and FAM is achieved, resulting in intensified green fluorescence upon ROS detection. Taken together, the FAM-C ₂₀/AgNCs-Cy5 redox sensor enables dynamic visualization of intracellular redox states, yielding insights into oxidative stress-related processes in live cells.

논문정보

형식Research article
게재일2024년 06월 (BRIC 등록일 2024-06-03)
연구진국내(교신)+국외 연구진
분야 생명과학 > 분자생물학

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