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Research, 2024 May 29:7:0388 | https://doi.org/10.34133/research.0388 pH-Responsive Theranostic Colloidosome Drug Carriers Enable Real-Time Imaging of Targeted Thrombolytic Process with Near-Infrared-II for Deep Venous Thrombosis

Yaxin Ye¹, Zhechang Chen¹, Shengzhang Zhang², Paul Slezak³, Fei Lu¹, Ruiqi Xie^1,3, Dongwon Lee^4*, Guangqian Lan^1*, and Enling Hu^1,5*

¹State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.

²Department of Cardiovascular Medicine, Yueqing People's Hospital, Wenzhou 325699, China.

³Ludwig Boltzmann Institute for Traumatology, AUVA Research Center, 1200 Vienna, Austria.

⁴Department of Bionanotechnology and Bioconvergence Engineering and Department of Polymer Nano Science and Technology, Jeonbuk National University, Jeonju, Chonbuk 54896, Republic of Korea.

⁵School of Fashion and Textiles, The Hong Kong Polytechnic University, Hong Kong.

^*Address correspondence to: Dongwon Lee, Guangqian Lan, and Enling Hu

Abstract

Thrombosis can cause life-threatening disorders. Unfortunately, current therapeutic methods for thrombosis using injecting thrombolytic medicines systemically resulted in unexpected bleeding complications. Moreover, the absence of practical imaging tools for thrombi raised dangers of undertreatment and overtreatment. This study develops a theranostic drug carrier, Pkr(IR-Ca/Pda-uPA)-cRGD, that enables real-time monitoring of the targeted thrombolytic process of deep vein thrombosis (DVT). Pkr(IR-Ca/Pda-uPA)-cRGD, which is prepared from a Pickering-emulsion-like system, encapsulates both near-infrared-II (NIR-II) contrast agent (IR-1048 dye, loading capacity: 28%) and urokinase plasminogen activators (uPAs, encapsulation efficiency: 89%), pioneering the loading of multiple drugs with contrasting hydrophilicity into one single-drug carrier. Upon intravenous injection, Pkr(IR-Ca/Pda-uPA)-cRGD considerably targets to thrombi selectively (targeting rate: 91%) and disintegrates in response to acidic thrombi to release IR-1048 dye and uPA for imaging and thrombolysis, respectively. Investigations indicate that Pkr(IR-Ca/Pda-uPA)-cRGD enabled real-time visualization of targeted thrombolysis using NIR-II imaging in DVT models, in which thrombi were eliminated (120 min after drug injection) without bleeding complications. This may be the first study using convenient NIR-II imaging for real-time visualization of targeted thrombolysis. It represents the precision medicine that enables rapid response to acquire instantaneous medical images and make necessary real-time adjustments to diagnostic and therapeutic protocols during treatment.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-31)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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