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가톨릭대학교 의과대학

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Emerg. Microbes Infect., May 29 2024, | https://doi.org/10.1080/22221751.2024.2362392 Characterization of Genotype V Japanese Encephalitis Virus Isolates from Republic of Korea

Ah-Ra Lee^a,b, Sang-Hyun Kim^a,b, Su-Yeon Hong^a,b, Sang-Ho Lee^a,b, Jae Sang Oh^c, KyungYong Lee^d, Seong-Jun Kim^e, Tomohiro Ishikawa^f, Sang-Mu Shim^g, Hee Il Lee^h, and Sang-Uk Seo^a,b*

^aDepartment of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, Seoul, Republic of Korea,

^bDepartment of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea,

^cDepartment of Neurosurgery, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea,

^dDivision of Cancer Biology, Research Institute, National Cancer Center, Goyang, Republic of Korea,

^eCenter for Infectious Disease Vaccine and Diagnosis Innovation(CEVI), Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea,

^fDepartment of Microbiology, Dokkyo Medical University School of Medicine, Tochigi, Japan,

^gDivision of Acute Virus Diseases, Korea National Institute of Health, Korea Disease Controland Prevention Agency, Cheongju, Republic of Korea,

^hDivision of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea.

^*Corresponding author: Sang-Uk Seo

Abstract

Japanese encephalitis (JE), caused by the Japanese encephalitis virus (JEV) infection, continues to pose significant public health challenges worldwide despite efficient vaccines. The virus is classified into five genotypes, among which genotype V (GV) was not detected for a long period after its initial isolation in 1952, until reports emerged from China and the Republic of Korea (ROK) since 2009. The characteristics of the virus are crucial in estimating its potential epidemiological impact. However, characterization of GV JEVs has so far been limited to two strains: Muar, the original isolate, and XZ0934, isolated in China. Two additional ROK GV JEV isolates, NCCP 43279 and NCCP 43413, are currently available, but their characteristics have not been explored. Our phylogenetic analysis revealed that GV virus sequences from the ROK segregate into two clades. NCCP 43279 and NCCP 43413 belong to different clades and exhibit distinct in vitro phenotypes. NCCP 43279 forms larger plaques but demonstrates inefficient propagation in cell culture compared to NCCP 43413. In vivo, NCCP 43279 induces higher morbidity and mortality in mice than NCCP 43413. Notably, NCCP 43279 shows more severe blood-brain barrier damage, suggesting superior brain invasion capabilities. Consistent with its higher virulence, NCCP 43279 displays more pronounced histopathological and immunopathological outcomes. In conclusion, our study confirms that the two ROK isolates are not only classified into different clades but also exhibit distinct in vitro and in vivo characteristics.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-30)
연구진국내(교신)+국외 연구진
분야 생명과학 > 미생물학

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