유웅재 (Vanderbilt University Medical Center, 현 POSTECH) | 한빛사논문 > 한빛사

한빛사논문

조회623

Vanderbilt University Medical Center, 현 POSTECH

Cell Host Microbe, May 27, 2024 | https://doi.org/10.1016/j.chom.2024.05.001 Salmonella Typhimurium expansion in the inflamed murine gut is dependent on aspartate derived from ROS-mediated microbiota lysis

Woongjae Yoo,^1,2,8 Nicolas G. Shealy,^1,8Jacob K. Zieba,¹ Teresa P. Torres,1 Madi Baltagulov,¹ Julia D. Thomas,¹ Catherine D. Shelton,¹ Anna G. McGovern,¹ Nora J. Foegeding,¹ Erin E. Olsan,³ and Mariana X. Byndloss ^{1,4,5,6,7,9,*}

¹Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
²Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
³Department of Biological Sciences, California State University, Sacramento, CA 95819, USA
⁴Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA
⁵Vanderbilt Digestive Disease Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA
⁶Vanderbilt Microbiome Innovation Center, Vanderbilt University, Nashville, TN 37235, USA
⁷Howard Hughes Medical Institute, Vanderbilt University Medical Center, Nashville, TN 37232, USA
⁸These authors contributed equally
⁹Lead contact

^*Corresponding author: correspondence to Mariana X. Byndloss

Abstract

Inflammation boosts the availability of electron acceptors in the intestinal lumen, creating a favorable niche for pathogenic Enterobacteriaceae. However, the mechanisms linking intestinal inflammation-mediated changes in luminal metabolites and pathogen expansion remain unclear. Here, we show that mucosal inflammation induced by Salmonella enterica serovar Typhimurium (S. Tm) infection increases intestinal levels of the amino acid aspartate. S. Tm used aspartate-ammonia lyase (aspA)-dependent fumarate respiration for growth in the murine gut only during inflammation. AspA-dependent growth advantage was abolished in the gut of germ-free mice and restored in gnotobiotic mice colonized with members of the classes Bacteroidia and Clostridia. Reactive oxygen species (ROS) produced during the host response caused lysis of commensal microbes, resulting in the release of microbiota-derived aspartate that was used by S. Tm, in concert with nitrate-dependent anaerobic respiration, to outcompete commensal Enterobacteriaceae. Our findings demonstrate the role of microbiota-derived amino acids in driving respiration-dependent S. Tm expansion during colitis.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-28)
연구진국내+국외 연구진
분야 생명과학 > 미생물학

댓글 작성 로그인

CV 열람하기

연락처 보기