정광철 (University of Florida)

한빛사논문

조회167

University of Florida

CV File 295 kb

J. Hazard. Mater., May 22 2024, 473 S0304-3894(24)01273-1 | https://doi.org/10.1016/j.jhazmat.2024.134694 A study at the wildlife-livestock interface unveils the potential of feral swine as a reservoir for extended-spectrum β-lactamase-producing Escherichia coli

Ting Liu ^a,b,1, Shinyoung Lee ^a,b,1, Miju Kim ^a,b,c, Peixin Fan^a,b,2, Raoul K. Boughton ^d, Christina Boucher ^e, Kwangcheol C. Jeong ^a,b*

^aEmerging Pathogens Institute, University of Florida, Gainesville, FL 32611 USA

^bDepartment of Animal Sciences, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL 32611 USA

^cDepartment of Food Science and Biotechnology, Kyung Hee University, Yongin, Republic of Korea

^dRange Cattle Research and Education Center, Wildlife Ecology and Conservation, University of Florida, Ona, FL 33865, USA

^eDepartment of Computer and Information Science and Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL 32611 USA

¹These authors contributed equally.

²Current affiliation: Department of Animal and Dairy Sciences, Mississippi State University.

^*Correspondence to: Kwangcheol C. Jeong

Abstract

Wildlife is known to serve as carriers and sources of antimicrobial resistance (AMR). Due to their unrestricted movements and behaviors, they can spread antimicrobial resistant bacteria among livestock, humans, and the environment, thereby accelerating the dissemination of AMR. Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is one of major concerns threatening human and animal health, yet transmission mechanisms at the wildlife-livestock interface are not well understood. Here, we investigated the mechanisms of ESBL-producing bacteria spreading across various hosts, including cattle, feral swine, and coyotes in the same habitat range, as well as from environmental samples over a two-year period. We report a notable prevalence and clonal dissemination of ESBL-producing E. coli in feral swine and coyotes, suggesting their persistence and adaptation within wildlife hosts. In addition, in silico studies showed that horizontal gene transfer, mediated by conjugative plasmids and insertion sequences elements, may play a key role in spreading the ESBL genes among these bacteria. Furthermore, the shared gut resistome of cattle and feral swine suggests the dissemination of antibiotic resistance genes at the wildlife-livestock interface. Taken together, our results suggest that feral swine may serve as a reservoir of ESBL-producing E. coli.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-27)
연구진국내+국외 연구진
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