이원재 (Duke University School of Medicine) | 한빛사논문 > 한빛사

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조회397

Duke University School of Medicine

Carbohydr. Polym., Available online 24 April 2024 | https://doi.org/10.1016/j.carbpol.2024.122204 Cell-adhesive double network self-healing hydrogel capable of cell and drug encapsulation: New platform to construct biomimetic environment with bottom-up approach

JaeWook Park ^a, Hyunji Kim ^a, Jeongwoo Ham ^a, Wonjae Lee ^b,c, Won-Gun Koh ^a

^aDepartment of Chemical and Biomolecular Engineering, Yonsei University, Seoul 03722, Republic of Korea

^bDepartments of Neurosurgery, Duke University School of Medicine, Durham, NC 27710, USA

^cDepartment of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA

Corresponding authors : Wonjae Lee, Won-Gun Koh

Abstract

This study presents the development and characterization of a novel double-network self-healing hydrogel based on N-carboxyethyl chitosan (CEC) and oxidized dextran (OD) with the incorporation of crosslinked collagen (CEC-OD/COL-GP) to enhance its biological and physicochemical properties. The hydrogel formed via dynamic imine bond formation exhibited efficient self-healing within 30 min, and a compressive modulus recovery of 92 % within 2 h. In addition to its self-healing ability, CEC-OD/COL-GP possesses unique physicochemical characteristics including transparency, injectability, and adhesiveness to various substrates and tissues. Cell encapsulation studies confirmed the biocompatibility and suitability of the hydrogel as a cell-culture scaffold, with the presence of a collagen network that enhances cell adhesion, spreading, long-term cell viability, and proliferation. Leveraging their unique properties, we engineered assemblies of self-healing hydrogel modules for controlled spatiotemporal drug delivery and constructed co-culture models that simulate angiogenesis in tumor microenvironments. Overall, the CEC-OD/COL-GP hydrogel is a versatile and promising material for biomedical applications, offering a bottom-up approach for constructing complex structures with self-healing capabilities, controlled drug release, and support for diverse cell types in 3D environments. This hydrogel platform has considerable potential for advancements in tissue engineering and therapeutic interventions.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-05-20)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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