이현수 (서강대학교)

한빛사논문

조회107

서강대학교

Chem. Rev., May 16 2024, | https://doi.org/10.1021/acs.chemrev.4c00112 Cellular and Biophysical Applications of Genetic Code Expansion

Han Bin Yi ¹, Seungeun Lee ¹, Kyungdeok Seo ¹, Hyeongjo Kim ¹, Minah Kim ¹, Hyun Soo Lee ¹

¹Department of Chemistry, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea.

Corresponding Author : Hyun Soo Lee

Abstract

Despite their diverse functions, proteins are inherently constructed from a limited set of building blocks. These compositional constraints pose significant challenges to protein research and its practical applications. Strategically manipulating the cellular protein synthesis system to incorporate novel building blocks has emerged as a critical approach for overcoming these constraints in protein research and application. In the past two decades, the field of genetic code expansion (GCE) has achieved significant advancements, enabling the integration of numerous novel functionalities into proteins across a variety of organisms. This technological evolution has paved the way for the extensive application of genetic code expansion across multiple domains, including protein imaging, the introduction of probes for protein research, analysis of protein-protein interactions, spatiotemporal control of protein function, exploration of proteome changes induced by external stimuli, and the synthesis of proteins endowed with novel functions. In this comprehensive Review, we aim to provide an overview of cellular and biophysical applications that have employed GCE technology over the past two decades.

논문정보

형식Review Paper
게재일2024년 05월 (BRIC 등록일 2024-06-18)
연구진국내 연구진
분야

댓글 작성 로그인

CV 열람하기

연락처 보기