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New Phytol., May 14 2024, | https://doi.org/10.1111/nph.19790 ATR2C ala2 from Arabidopsis-infecting downy mildew requires 4 TIR-NLR immune receptors for full recognition

Dae Sung Kim ^1,2, Yufei Li ², Hee-Kyung Ahn ², Alison Woods-Tör ³, Volkan Cevik⁴, Oliver J. Furzer ², Wenbo Ma ², Mahmut Tör ³, Jonathan D. G. Jones ²

¹State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, 430062, China.

²The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.

³Department of Biological Sciences, School of Science and the Environment, University of Worcester, Worcester, WR2 6AJ, UK.

⁴Department of Life Sciences, The Milner Centre for Evolution, University of Bath, Bath, BA2 7AY, UK.

CORRESPONDING AUTHORS : Mahmut Tör, Jonathan D. G. Jones

Abstract

Arabidopsis Col-0 RPP2A and RPP2B confer recognition of Arabidopsis downy mildew (Hyaloperonospora arabidopsidis [Hpa]) isolate Cala2, but the identity of the recognized ATR2 ^Cala2 effector was unknown. To reveal ATR2 ^Cala2, an F ₂ population was generated from a cross between Hpa-Cala2 and Hpa-Noks1. We identified ATR2 ^Cala2 as a non-canonical RxLR-type effector that carries a signal peptide, a dEER motif, and WY domains but no RxLR motif. Recognition of ATR2 ^Cala2 and its effector function were verified by biolistic bombardment, ectopic expression and Hpa infection. ATR2 ^Cala2 is recognized in accession Col-0 but not in Ler-0 in which RPP2A and RPP2B are absent. In ATR2 ^Emoy2 and ATR2 ^Noks1 alleles, a frameshift results in an early stop codon. RPP2A and RPP2B are essential for the recognition of ATR2 ^Cala2. Stable and transient expression of ATR2 ^Cala2 under 35S promoter in Arabidopsis and Nicotiana benthamiana enhances disease susceptibility. Two additional Col-0 TIR-NLR (TNL) genes (RPP2C and RPP2D) adjacent to RPP2A and RPP2B are quantitatively required for full resistance to Hpa-Cala2. We compared RPP2 haplotypes in multiple Arabidopsis accessions and showed that all four genes are present in all ATR2 ^Cala2-recognizing accessions.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-17)
연구진국외 연구진
분야 생명과학 > 식량작물 및 원예작물

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