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Biosens. Bioelectron., May 09 2024, 259 S0956-5663(24)00380-4 | https://doi.org/10.1016/j.bios.2024.116375 Dual structure-switching aptamer-mediated signal amplification cascade for SARS-CoV-2 detection

Jaewoo Lim ^a,b, Seong Uk Son ^a,c, Jisun Ki ^a, Sunjoo Kim ^d, Jina Lee ^a,c, Soojin Jang ^a,c, Seung Beom Seo ^a,e, Hyowon Jang ^a, Taejoon Kang ^a,f, Juyeon Jung ^a,c,f, Eunjung Kim ^g,h, Eun-Kyung Lim ^a,c,f

^aBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea

^bMedical Device Development Center, Osong Medical Innovation Foundation, 123 Osongsaengmyeong-ro, Chungcheongbuk-do, 28160, Republic of Korea

^cDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology 125 Gwahak-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea

^dDepartment of Laboratory Medicine, Gyeongsang National University Changwon Hospital, Changwon, 51472, Republic of Korea

^eDepartment of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea

^fSchool of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea

^gDepartment of Bioengineering & Nano-bioengineering, Research Center for Bio Materials and Process Development, Incheon National University, Incheon, 22012, Republic of Korea

^hDivision of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

Corresponding author : Eunjung Kim, Eun-Kyung Lim

Abstract

Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) at the end of 2019, the spread of the virus has posed a significant threat to public health and the global economy. This work proposed a one-step, dual-structure-switching aptamer-mediated signal amplification cascade for rapid and sensitive detection of the SARS-CoV-2 nucleocapsid protein. This system consisted of two DNA aptamers with structure-switching functionality and fuel DNA, where a cascade of strand hybridization and displacement triggered fluorescence generation and signal amplification. This aptamer-based amplification cascade required neither an amplification stage using enzymes nor pre-processing steps such as washing, viral isolation, and gene extraction. The assay could distinguish SARS-CoV-2 from other respiratory viruses and detect up to 1.0 PFU/assay of SARS-CoV-2 within 30 min at room temperature. In 35 nasopharyngeal clinical samples, the assay accurately assessed 25 positive and 10 negative clinical swab samples, which were confirmed using quantitative polymerase chain reaction. The strategy reported herein can help detect newly emerging pathogens and biomarkers of various diseases in liquid samples. In addition, the developed detection system consisting of only DNA and fluorophores can be widely integrated into liquid biopsy platforms for disease diagnosis.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-21)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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