민지희 (전남대학교, Texas A&M University, 현 University of Michigan) | 한빛사논문 > 한빛사

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전남대학교, Texas A&M University, 현 University of Michigan

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Plant J., May 14 2024, | https://doi.org/10.1111/tpj.16808 Rhamnogalacturonan lyase 1 (RGL1), as a suppressor of E3 ubiquitin ligase Arabidopsis thaliana ring zinc finger 1 (AtRZF1), is involved in dehydration response to mediate proline synthesis and pectin rhamnogalacturonan-I composition

Ji-Hee Min^1,2,†, Cho-Rong Park¹, Ying Gong¹, Moon-Soo Chung³, Seung-Hee Nam⁴, Hye Sup Yun⁵ and Cheol Soo Kim^1,*

¹Department of Applied Biology, Chonnam National University, Gwangju 61186 Republic of Korea,

²Department of Biochemistry and Biophysics, Texas A&M University, 300 Olsen Blvd, College Station, Texas 77843-2128 USA,

³Research Division for Radiation Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy ResearchInstitute, Jeongeup-si, Jeonlabuk-do 56212 Republic of Korea,

⁴Institute of Agricultural Science and Technology, Chonnam National University, Gwangju 61186 Republic of Korea,

⁵Department of Biological Sciences, Konkuk University, Seoul 05029 Republic of Korea

^*For correspondence : Cheol Soo Kim

^†Present address: Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109 USA

Abstract

Proline metabolism plays a crucial role in both environmental stress responses and plant growth. However, the specific mechanism by which proline contributes to abiotic stress processes remains to be elucidated. In this study, we utilized atrzf1 (Arabidopsis thaliana ring zinc finger 1) as a parental line for T-DNA tagging mutagenesis and identified a suppressor mutant of atrzf1, designated proline content alterative 31 (pca31). The pca31 mutant suppressed the insensitivity of atrzf1 to dehydration stress during early seedling growth. Using Thermal Asymmetric Interlaced-PCR, we found that the T-DNA of pca31 was inserted into the promoter region of the At2g22620 gene, which encodes the cell wall enzyme rhamnogalacturonan lyase 1 (RGL1). Enzymatic assays indicated that RGL1 exhibited rhamnogalacturonan lyase activity, influencing cell wall pectin composition. The decrease in RGL1 gene expression suppressed the transcriptomic perturbation of the atrzf1 mutant. Silencing of the RGL1 gene in atrzf1 resulted in a sensitive phenotype similar to pca31 under osmotic stress conditions. Treatment with mannitol, salt, hydrogen peroxide, and abscisic acid induced RGL1 expression. Furthermore, we uncovered that RGL1 plays a role in modulating root growth and vascular tissue development. Molecular, physiological, and genetic experiments revealed that the positive modulation of RGL1 during abiotic stress was linked to the AtRZF1 pathway. Taken together, these findings establish that pca31 acts as a suppressor of atrzf1 in abiotic stress responses through proline and cell wall metabolisms.

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형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-17)
연구진국내(교신)+국외 연구진
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