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Manorma NegiManorma Negi

광운대학교

J. Hazard. Mater., May 10 2024, 472 S0304-3894(24)01141-5 | https://doi.org/10.1016/j.jhazmat.2024.134562 Biocompatible plasma-treated liquids: A sustainable approach for decontaminating gastrointestinal-infection causing pathogens

Manorma Negi ^a, Neha Kaushik ^b, Prajwal Lamichhane ^a, Apurva Jaiswal ^a, Shweta B. Borkar ^a, Paritosh Patel ^a, Prashant Singh ^c, Eun Ha Choi ^a, Nagendra Kumar Kaushik^a

^aPlasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul 01897, South Korea

^bDepartment of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, South Korea

^cDepartment of Chemistry, Atma Ram Sanatan Dharma College, University of Delhi, Delhi, India

Corresponding authors : Neha Kaushik, Eun Ha Choi, Nagendra Kumar Kaushik

Abstract

Nosocomial infections are a serious threat and difficult to cure due to rising antibiotic resistance in pathogens and biofilms. Direct exposure to cold atmospheric plasma (CAP) has been widely employed in numerous biological research endeavors. Nonetheless, plasma-treated liquids (PTLs) formulated with physiological solutions may offer additional benefits such as enhanced portability, and biocompatibility. Additionally, CAP-infused long-lived reactive oxygen and nitrogen species (RONS) such as nitrite (NO ₂ ^-), nitrate (NO ₃ ^-), and hydrogen peroxide (H ₂O ₂) can synergistically induce their antibacterial activity. Herein, we investigated those argon-plasma jet-treated liquids, including Ringer's lactate (RL), phosphate-buffered saline (PBS), and physiological saline, have significant antibacterial activity against nosocomial/gastrointestinal-causing pathogens, which might be due to ROS-mediated lipid peroxidation. Combining the conventional culture-based method with propidium iodide monoazide quantitative PCR (PMAxx™-qPCR) indicated that PTLs induce a minimal viable but non-culturable (VBNC) state and moderately affect culturable counts. Specifically, the PTL exposure resulted in pathogenicity dysfunction via controlling T3SS-related effector genes of S. enterica. Overall, this study provides insights into the effectiveness of PTLs for inducing ROS-mediated damage, controlling the virulence of diarrheagenic bacteria, and modulating homeostatic genes.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-16)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오소재

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