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조회273

소정옥Jeong Ook Soh

한양대학교

ACS Nano, May 11 2024, | https://doi.org/10.1021/acsnano.3c12266 Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes

Bum Chul Park¹, Jeong Ook Soh ^2,3, Hee-Joo Choi ^4,5,6, Hyeon Su Park ⁷, Sang Min Lee^2,3, Hong En Fu ⁷, Myeong Soo Kim⁷, Min Jun Ko⁸, Thomas Myeongseok Koo ⁷, Jeong-Yeon Lee ^4,5, Young Keun Kim ^7,9, Ju Hun Lee ^2,3

¹Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109-2136, United States.

²Department of Bionano Engineering, Hanyang University, Ansan 15588, Republic of Korea.

³Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 15588, Republic of Korea.

⁴Department of Pathology, Hanyang University, Seoul 04763, Republic of Korea.

⁵Hanyang Institute of Bioscience and Biotechnology (HY-IBB), Hanyang University, Seoul 04763, Republic of Korea.

⁶Hanyang Biomedical Research Institute (HBRI), Hanyang University, Seoul 04763, Republic of Korea.

⁷Department of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea.

⁸Department of Radiology, Northwestern University, Chicago, Illinois 60611, United States.

⁹Brain Korea Center for Smart Materials and Devices, Korea University, Seoul 02841, Republic of Korea.

B.C.P., J.O.S., H.-J.C., H.S.P., and S.M.L. contributed equally to the work.

Corresponding Authors : Jeong-Yeon Lee, Young Keun Kim, Ju Hun Lee

Abstract

Circulating tumor DNA (ctDNA) detection has been acknowledged as a promising liquid biopsy approach for cancer diagnosis, with various ctDNA assays used for early detection and treatment monitoring. Dispersible magnetic nanoparticle-based electrochemical detection methods have been proposed as promising candidates for ctDNA detection based on the detection performance and features of the platform material. This study proposes a nanoparticle surface-localized genetic amplification approach by integrating Fe ₃O ₄-Au core-shell nanoparticles into polymerase chain reactions (PCR). These highly dispersible and magnetically responsive superparamagnetic nanoparticles act as nano-electrodes that amplify and accumulate target ctDNA in situ on the nanoparticle surface upon PCR amplification. These nanoparticles are subsequently captured and subjected to repetitive electrochemical measurements to induce reconfiguration-mediated signal amplification for ultrasensitive (∼3 aM) and rapid (∼7 min) metastatic breast cancer ctDNA detection in vitro. The detection platform can also detect metastatic biomarkers from in vivo samples, highlighting the potential for clinical applications and further expansion to rapid and ultrasensitive multiplex detection of various cancers.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-14)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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