김병후 (중앙대학교)

한빛사논문

조회165

김병후Byoung Hu Kim

중앙대학교

Int. J. Antimicrob. Agents, May 07 2024, S0924-8579(24)00112-2 | https://doi.org/10.1016/j.ijantimicag.2024.107194 Isolation, characterization, and application of bacteriophage on Vibrio parahaemolyticus biofilm to control seafood contamination

Byoung Hu Kim ^a,†, Md. Ashrafudoulla^a,b,†, Shanjida Shaila ^a, Hyung Jin Park ^c, Jeong Dug Sul ^c, Si Hong Park ^d, Sang-Do Ha ^a

^aFood Science and Technology, Chung-Ang University, Republic of Korea

^bCurrent position: National Eye Institute, NIH, Bethesda, MD 20892, USA

^cCollege of Sport Sciences, Chung-Ang University, Republic of Korea

^dFood Science and Technology, Oregon State University, USA

^†Contributed equally.

Corresponding author: Sang-Do Ha

Abstract

This study intended to isolate a Vibrio-particular phage from the natural environment, analyze its characteristics and genome sequence, and investigate its reduction effect on V. parahaemolyticus biofilm as a biocontrol agent in squid and mackerel. Among 21 phages, phage CAU_VPP01, isolated from beach mud, was chosen for further experiments based on host range and EOP tests. When examining the reduction effect of phage CAU_VPP01 against Vibrio parahaemolyticus biofilms on surfaces (stainless steel [SS] and polyethylene terephthalate [PET]) and food surfaces (squid and mackerel), the phage showed the most excellent reduction effect at a multiplicity-of-infection (MOI) 10. Three-dimensional images acquired with confocal laser scanning microscopy (CLSM) analysis were quantified using COMSTAT, which showed that biomass, average thickness, and roughness coefficient decreased when treated with the phage. Color and texture analysis confirmed that the quality of squid and mackerel was maintained after the phage treatment. Finally, a comparison of gene expression levels determined by qRT-PCR analysis showed that the phage treatment induced a decrease in the gene expression of flaA, vp0962, and luxS, as examples. This study indicated that Vibrio-specific phage CAU_VPP01 effectively controlled V. parahaemolyticus biofilms under various conditions and confirmed that the isolated phage could possibly be used as an effective biocontrol weapon in the seafood manufacturing industry.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-16)
연구진국내 연구진
분야 생명과학 > 식품학

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