박재성 (POSTECH, University of Michigan)

한빛사논문

조회123

POSTECH, University of Michigan

CV File 56 kb

J. Extracell. Vesicles, Apr 2024, 13 (4) e12438 | https://doi.org/10.1002/jev2.12438 Cell-engineered virus-mimetic nanovesicles for vaccination against enveloped viruses

Chungmin Han¹, Suyeon Kim¹, Youngjoo Seo¹, Minyeob Lim¹, Yongmin Kwon², Johan Yi², Seung-Ik Oh¹, Minsu Kang¹, Seong Gyu Jeon³, Jaesung Park^1,2,4

¹School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea

²Department of Mechanical Engineering, Pohang University of Science and Technology(POSTECH), Pohang, Republic of Korea

³Exosome Plus, Suwon, Republic of Korea

⁴Wireless Integrated MicroSensing & Systems, College of Engineering, University of Michigan, Ann Arbor, Michigan, USA

Correspondence: Jaesung Park

Abstract

Enveloped viruses pose a significant threat to human health, as evidenced by the recent COVID-19 pandemic. Although current vaccine strategies have proven effective in preventing viral infections, the development of innovative vaccine technologies is crucial to fortify our defences against future pandemics. In this study, we introduce a novel platform called cell-engineered virus-mimetic nanovesicles (VNVs) and demonstrate their potential as a vaccine for targeting enveloped viruses. VNVs are generated by extruding plasma membrane-derived blebs through nanoscale membrane filters. These VNVs closely resemble enveloped viruses and extracellular vesicles (EVs) in size and morphology, being densely packed with plasma membrane contents and devoid of materials from other membranous organelles. Due to these properties, VNVs express viral membrane antigens more extensively and homogeneously than EVs expressing the same antigen. In this study, we produced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) VNVs expressing the SARS-CoV-2 Spike glycoprotein (S) on their surfaces and assessed their preclinical efficacy as a COVID-19 vaccine in experimental animals. The administration of VNVs successfully stimulated the production of S-specific antibodies both systemically and locally, and immune cells isolated from vaccinated mice displayed cytokine responses to S stimulation.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-07-04)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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