조규봉 (서강대학교)

한빛사논문

조회228

서강대학교

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Adv. Sci., May 05 2024, | https://doi.org/10.1002/advs.202309702 Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal-Free Electro-Stain Composed of DNA-Binding Proteins and Synthetic Polymers

Chanyoung Noh ¹, Yoonjung Kang ¹, Sujung Heo ¹, Taesoo Kim ¹, Hayeon Kim ¹, Junhyuck Chang ², Priyannth Ramasami Sundharbaabu ², Sanghee Shim³, Kwang-Il Lim ⁴, Jung Heon Lee ^{2 *}, Kyubong Jo ^{1 *}

¹Department of Chemistry, Sogang University, Seoul, 04107, South Korea.

²School of Advanced Materials Science and Engineering, Department of MetaBioHealth Sungkyunkwan University (SKKU), Suwon, 16419, South Korea.

³Department of Chemistry, Korea University, Seoul, 02841, South Korea.

⁴Department of Chemical and Biological Engineering, Sookmyung Women's University, Seoul, 04312, South Korea.

^*Corresponding authors: correspondence to Jung Heon Lee or Kyubong Jo

Abstract

This paper presents the first scanning electron microscopy (SEM)-based DNA imaging in biological samples. This novel approach incorporates a metal-free electro-stain reagent, formulated by combining DNA-binding proteins and synthetic polymers to enhance the visibility of 2-nm-thick DNA under SEM. Notably, DNA molecules stain with proteins and polymers appear as dark lines under SEM. The resulting DNA images exhibit a thickness of 15.0±4.0 nm. As SEM is the primary platform, it integrates seamlessly with various chemically functionalized large surfaces with the aid of microfluidic devices. The approach allows high-resolution imaging of various DNA structures including linear, circular, single-stranded DNA and RNA, originating from nuclear and mitochondrial genomes. Furthermore, quantum dots are successfully visualized as bright labels that are sequence-specifically incorporated into DNA molecules, which highlights the potential for SEM-based optical DNA mapping. In conclusion, DNA imaging using SEM with the novel electro-stain offers electron microscopic resolution with the ease of optical microscopy.

논문정보

형식Research article
게재일2024년 05월 (BRIC 등록일 2024-05-07)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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