한빛사논문
- 조회303
A Hyeon Kim a,1, Seungho Jeon b,1, Kaustuv Roy a, Tae Min Kim a, Eun-Jung Jin b, Sung Young Park a,c
aDepartment of IT and Energy Convergence (BK21 FOUR), Korea National University of Transportation, Chungju 27469, Republic of Korea
bDepartment of Biological Sciences, College of Health Sciences, Wonkwang University, Iksan, Jeonbuk 54538, Korea
cDepartment of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 27469, Republic of Korea
1These authors are equally contributed.
Corresponding authors : Eun-Jung Jin, Sung Young Park
Abstract
A redox-responsive low-viscosity, injectable hydrogel (LVI-gel) sensor with capabilities involving bimodal cancer detection via the incorporation of glutathione (GSH) and reactive oxygen species (ROS)-activated nanoparticles (PD-Cu2+) is proposed and made injectable with varying degrees of mineralization. The PD-Cu2+ complex, in the presence of overexpressed GSH and ROS, undergoes a structural change that causes a regain of fluorescence and a shift in conductivity, accompanied by the variations in the mechanical, and adhesive properties for cancer detection. The release of catechol moieties brought about by the structural change of the PD-Cu2+ complex influences the shift in hydrogen bonding, resulting in increased water uptake, further reducing viscosity (η decreases by 95.14 %) and increasing adhesiveness (360.28 % increase) in the presence of GSH 10 mM. The alterations in mechanical and electrical properties further affect the bending response via LCR (control: ΔR/R0 ∼ 0.55 and GSH 10 mM: ΔR/R0 ∼ 0.89), which was confirmed in vitro via wireless response with HeLa, PC-3, and B16F10 as cancer cells. The effect of Cu2+-catalyzed oxidation of GSH was observed in vivo from the immunohistochemical studies and the downregulation of transcription for GPx1/Rn18s. Additionally, increased superoxide dismutase (SOD2) and catalase (CAT) activity along with the downregulation of the oncogenic NFκβ and TNF gene are observed. Finally, the ex situ sensing activity was monitored by the shift in resistance and the wireless response, demonstrating the ease with which the sensor could diagnose.
논문정보
- Research article
- 2024년 06월 (BRIC 등록일 2024-05-07)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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