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김미진Mijin Kim

Sloan Kettering Institute, Georgia Institute of Technology

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J. Am. Chem. Soc., Apr 30 2024, | https://doi.org/10.1021/jacs.4c00149 Quantum Defect Sensitization via Phase-Changing Supercharged Antibody Fragments

Mijin Kim ^1,2, James J McCann ³, Jacob Fortner ^4,5, Ewelina Randall ¹, Chen Chen ^1,6, Yu Chen ³, Zvi Yaari ^1,7, YuHuang Wang ^4,5, Ronald L Koder^3,8, Daniel A Heller ^1,6

¹Molecular Pharmacology Program, Sloan Kettering Institute, New York, New York 10065, United States.

²School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

³Department of Physics, City College of New York, New York, New York 10031, United States.

⁴Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, United States.

⁵Chemical Physics Program, University of Maryland, College Park, Maryland 20742, United States.

⁶Graduate School of Medical Sciences, Weill Cornell Medicine, New York, New York 10065, United States.

⁷School of Pharmacy, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9190500, Israel.

⁸Graduate Programs of Physics, Biology, Chemistry, and Biochemistry, The Graduate Center of City College of New York, New York, New York 10016, United States.

Corresponding Authors : Ronald L. Koder, Daniel A. Heller

Abstract

Quantum defects in single-walled carbon nanotubes promote exciton localization, which enables potential applications in biodevices and quantum light sources. However, the effects of local electric fields on the emissive energy states of quantum defects and how they can be controlled are unexplored. Here, we investigate quantum defect sensitization by engineering an intrinsically disordered protein to undergo a phase change at a quantum defect site. We designed a supercharged single-chain antibody fragment (scFv) to enable a full ligand-induced folding transition from an intrinsically disordered state to a compact folded state in the presence of a cytokine. The supercharged scFv was conjugated to a quantum defect to induce a substantial local electric change upon ligand binding. Employing the detection of a proinflammatory biomarker, interleukin-6, as a representative model system, supercharged scFv-coupled quantum defects exhibited robust fluorescence wavelength shifts concomitant with the protein folding transition. Quantum chemical simulations suggest that the quantum defects amplify the optical response to the localization of charges produced upon the antigen-induced folding of the proteins, which is difficult to achieve in unmodified nanotubes. These findings portend new approaches to modulate quantum defect emission for biomarker sensing and protein biophysics and to engineer proteins to modulate binding signal transduction.

논문정보

형식Research article
게재일2024년 04월 (BRIC 등록일 2024-05-03)
연구진국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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